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Anti-KDM5B / PLU1 / Jarid1B antibody [JARIA3D4] (ab50775)

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Overview

Product name

Anti-KDM5B / PLU1 / Jarid1B antibody [JARIA3D4]
See all KDM5B / PLU1 / Jarid1B products (7) ...

Description

Mouse monoclonal [JARIA3D4] to KDM5B / PLU1 / Jarid1B

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant Human JARID1B

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, 8mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 µm membrane.

Clonality

Monoclonal

Clone number

JARIA3D4

Isotype

IgG1

Research areas

Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors

  • Western blot - PLU1 / Jarid1B antibody [JARIA3D4] (ab50775)Western blot - PLU1 / Jarid1B antibody [JARIA3D4] (ab50775) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab50775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: Use at an assay depe...Read more →

    WB: Use at an assay dependent dilution. Detects a band of approximately 45 kDa (predicted molecular weight: 190 kDa).

Target

Function

Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma.

Tissue specificity

Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level).

Sequence similarities

Belongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 3 PHD-type zinc fingers.

Domain

Both the JmjC domain and the JmjN domain are required for enzymatic activity.
The 2 first PHD-type zinc finger domains are required for transcription repression activity.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ9UGL1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Cancer/testis antigen 31 antibody
  • CT31 antibody
  • Histone demethylase JARID1B antibody
  • JARID1B antibody
  • Jumonji AT rich interactive domain 1B antibody
  • Jumonji/ARID domain-containing protein 1B antibody
  • Kdm5b antibody
  • KDM5B_HUMAN antibody
  • Lysine specific demethylase 5B antibody
  • Lysine-specific demethylase 5B antibody
  • PLU-1 antibody
  • PUT1 antibody
  • RB-binding protein antibody
  • rbbp2h1a antibody
  • RBP2 like antibody
  • RBP2-H1 antibody
  • Retinoblastoma-binding protein 2 homolog 1 antibody
see all

Database links

Anti-KDM5B / PLU1 / Jarid1B antibody [JARIA3D4] images:

  Western blot - PLU1 / Jarid1B antibody [JARIA3D4] (ab50775)

Western blot - PLU1 / Jarid1B antibody [JARIA3D4] (ab50775)

Anti-KDM5B / PLU1 / Jarid1B antibody [JARIA3D4] (ab50775) + Immunizing recombinant fragment

Predicted band size : 190 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)

References for Anti-KDM5B / PLU1 / Jarid1B antibody [JARIA3D4] (ab50775)

ab50775 has not yet been referenced specifically in any publications.

Publishing research using ab50775? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"