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Products:Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Overview
Product name
Anti-KDM5B / PLU1 / Jarid1B antibody
See all KDM5B / PLU1 / Jarid1B products (7) ...
Description
Mouse monoclonal to KDM5B / PLU1 / Jarid1B
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: AKRMRAEAMN IKIEPEETTE ARTHNLRRRM GCPTPKCENE KEMKSSIKQE PIERKDYIVE NEKEKPKSRS KKATNAVDLY VCLLCGSGN, corresponding to amino acids 231-320 of Human PLU1 / Jarid1B
AKRMRAEAMN IKIEPEETTE ARTHNLRRRM GCPTPKCENE KEMKSSIKQE PIERKDYIVE NEKEKPKSRS KKATNAVDLY VCLLCGSGN
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab56759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 190 kDa.
IHC-P: Use a concentration of 1 µg/ml
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma.
Tissue specificity
Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level).
Sequence similarities
Belongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 3 PHD-type zinc fingers.
Domain
Both the JmjC domain and the JmjN domain are required for enzymatic activity.
The 2 first PHD-type zinc finger domains are required for transcription repression activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9UGL1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cancer/testis antigen 31 antibody
- CT31 antibody
- Histone demethylase JARID1B antibody
see all
Database links
- Entrez Gene: 10765 Human
- Omim: 605393 Human
- SwissProt: Q9UGL1 Human
- Unigene: 443650 Human
Anti-KDM5B / PLU1 / Jarid1B antibody images:
IHC-P - PLU1 / Jarid1B antibody (ab56759)
PLU1 / Jarid1B antibody (ab56759) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human colon.
Western blot - KDM5B / PLU1 / Jarid1B antibody (ab56759)
Predicted band size : 190 kDa
PLU1 / Jarid1B antibody (ab56759) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Western blot - KDM5B / PLU1 / Jarid1B antibody (ab56759)
All lanes : Anti-KDM5B / PLU1 / Jarid1B antibody (ab56759) at 1/500 dilution
Lane 1 : Human Pancreatic Cancer whole cell lysate (Line AsPc1)
Lane 2 : Human Pancreatic Cancer whole cell lysate (line Panc1)
Lane 3 : Human Pancreatic Cancer whole cell lysate (Line CaPan1)
Lysates/proteins at 35 µg per lane.
Secondary
An HRP-conjugated Sheep anti-mouse polyclonal at 1/5000 dilution
developed using the ECL technique
Performed under non-reducing conditions.
Predicted band size : 190 kDa
Observed band size : 190 kDa
Exposure time : 15 minutes
Blocking Step: 10% Milk for 1 hour at room temperature
This image is courtesy of an Abreview submitted by Pawel Mazur
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KDM5B / PLU1 / Jarid1B antibody (ab56759)
ab56759 staining KDM5B/PLU1/Jarid1B in Human pancreatic cancer tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with PBS + Triton 0.025% and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/100 in PBS) for 8 hours at 4°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Immunocytochemistry/ Immunofluorescence-KDM5B / PLU1 / Jarid1B antibody(ab56759)
ICC/IF image of ab56759 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26759, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-KDM5B / PLU1 / Jarid1B antibody (ab56759)
ab56759 has not yet been referenced specifically in any publications.
Publishing research using ab56759? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"