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Anti-KDM5B / PLU1 / Jarid1B antibody (ab84883)

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Overview

Product name

Anti-KDM5B / PLU1 / Jarid1B antibody
See all KDM5B / PLU1 / Jarid1B products (7) ...

Description

Rabbit polyclonal to KDM5B / PLU1 / Jarid1B

Tested applications

IHC-Pmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide corresponding to a region between residue 1494 and 1544 of human KDM5B/ PLU1/ Jarid1B (accession number: NP_006609.3)

Positive control

Human Controls: Breast Carcinoma, Prostate Carcinoma

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified using an epitope specific to KDM5B / PLU1 / Jarid1B immobilized on solid support.

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KDM5B / PLU1 / Jarid1B antibody (ab84883)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KDM5B / PLU1 / Jarid1B antibody (ab84883) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KDM5B / PLU1 / Jarid1B antibody (ab84883)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KDM5B / PLU1 / Jarid1B antibody (ab84883) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab84883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma.

Tissue specificity

Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level).

Sequence similarities

Belongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 3 PHD-type zinc fingers.

Domain

Both the JmjC domain and the JmjN domain are required for enzymatic activity.
The 2 first PHD-type zinc finger domains are required for transcription repression activity.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ9UGL1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Cancer/testis antigen 31 antibody
  • CT31 antibody
  • Histone demethylase JARID1B antibody
  • JARID1B antibody
  • Jumonji AT rich interactive domain 1B antibody
  • Jumonji/ARID domain-containing protein 1B antibody
  • KDM5B antibody
  • KDM5B_HUMAN antibody
  • Lysine specific demethylase 5B antibody
  • Lysine-specific demethylase 5B antibody
  • PLU-1 antibody
  • PUT1 antibody
  • RBBP2H1A antibody
  • RBP2 like antibody
  • RBP2-H1 antibody
  • Retinoblastoma-binding protein 2 homolog 1 antibody
see all

Database links

Anti-KDM5B / PLU1 / Jarid1B antibody images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KDM5B / PLU1 / Jarid1B antibody (ab84883)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KDM5B / PLU1 / Jarid1B antibody (ab84883)

Detection of Human Jarid1B in a formalin-fixed paraffin-embedded section of human prostate carcinoma by Immunohistochemistry using ab84883 at a dilution of 1:500. Detection: DAB staining.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KDM5B / PLU1 / Jarid1B antibody (ab84883)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KDM5B / PLU1 / Jarid1B antibody (ab84883)

Detection of Human Jarid1B in a formalin-fixed paraffin-embedded section of human breast carcinoma by Immunohistochemistry using ab84883 at a dilution of 1:250. Detection: DAB staining.

References for Anti-KDM5B / PLU1 / Jarid1B antibody (ab84883)

ab84883 has not yet been referenced specifically in any publications.

Publishing research using ab84883? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"