If your product does not perform as described on this datasheet, we will refund or replace your product...
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Would like to use in ChIP with mouse samples. |
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ANSWER: |
Thanks for your call today and for your interest in our KDM5C antibodies.
Since ab72152 has not been tested against mouse samples in ChIP, I can offer a discount off a future purchase if you buy ab72152 now, test it in ChIP with mouse samples, and submit feedback to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody.
If you are interested in this offer, please follow these steps:
1. Reply to this e-mail to let me know that you would like to proceed and test ab72152 in mouse. I will then send a discount code. This code must be issued before purchasing ab72152 so please wait for my reply before ordering.
2. Purchase ab72152 either by phone, fax, or online (www.abcam.com).
3. Test it in ChIP with mouse samples.
4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: http://www.abcam.com/abreviews.
5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab72152 turns out to be unsuitable for this application, you will still receive the discount on your next purchase after your Abreview has been submitted.
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-KDM5C / Jarid1C / SMCX antibody (ab72152) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size : 176 kDa
Observed band size : 200 kDa (why is the actual band size different from the predicted?)
Additional bands at : 220 kDa,300 kDa. We are unsure as to the identity of these extra bands.
Detection of Human KDM5C / Jarid1C / SMCX by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg/IP, 20% of IP loaded) using ab72152 at 3 µg/mg lysate for IP and at 0.1 µg/ml for subsequent WB detection. Note: bands between 55 and 71 kDa represent the IgG chains.
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