Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Overview
Product name
Anti-KDM5C / Jarid1C / SMCX antibody
See all KDM5C / Jarid1C / SMCX products (3) ...
Description
Rabbit polyclonal to KDM5C / Jarid1C / SMCX
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to a region between residues 275 and 325 of human KDM5C / Jarid1C / SMCX (NP_004178.2).
Positive control
HeLa whole cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IP: Use at 2 - 5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 176 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements.
Tissue specificity
Expressed in all tissues examined. Highest levels found in brain and skeletal muscle.
Involvement in disease
Defects in KDM5C are the cause of mental retardation syndromic X-linked JARID1C-related (MRXSJ) [MIM:300534]. MRXSJ is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXSJ patients manifest mental retardation associated with variable features such as slowly progressive spastic paraplegia, seizures, facial dysmorphism.
Sequence similarities
Belongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 2 PHD-type zinc fingers.
Domain
The first PHD-type zinc finger domain recognizes and binds H3-K9Me3.
Both the JmjC domain and the JmjN domain are required for enzymatic activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP41229
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- SmcX protein antibody
- Xe169 protein antibody
- DXS1272E antibody
see all
Database links
- Entrez Gene: 8242 Human
- Omim: 314690 Human
- SwissProt: P41229 Human
- SwissProt: Q7Z5S5 Human
- SwissProt: Q86WW4 Human
- Unigene: 631768 Human
Anti-KDM5C / Jarid1C / SMCX antibody images:
Western blot - KDM5C / Jarid1C / SMCX antibody (ab72152)
All lanes : Anti-KDM5C / Jarid1C / SMCX antibody (ab72152) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size : 176 kDa
Observed band size : 200 kDa (why is the actual band size different from the predicted?)
Additional bands at : 220 kDa,300 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - KDM5C / Jarid1C / SMCX antibody (ab72152)
Detection of Human KDM5C / Jarid1C / SMCX by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg/IP, 20% of IP loaded) using ab72152 at 3 µg/mg lysate for IP and at 0.1 µg/ml for subsequent WB detection. Note: bands between 55 and 71 kDa represent the IgG chains.
References for Anti-KDM5C / Jarid1C / SMCX antibody (ab72152)
ab72152 has not yet been referenced specifically in any publications.
Publishing research using ab72152? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"