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Western blot - KDM5C / Jarid1C / SMCX antibody (ab72152)
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Immunoprecipitation - KDM5C / Jarid1C / SMCX antibody (ab72152)
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Product Name
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KDM5C / Jarid1C / SMCX antibody
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See all KDM5C / Jarid1C / SMCX antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to KDM5C / Jarid1C / SMCX
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Immunogen
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Synthetic peptide corresponding to a region between residues 275 and 325 of human KDM5C / Jarid1C / SMCX (NP_004178.2).
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at 2 - 5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 176 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa whole cell lysate.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Chromatin and Nuclear Signaling >> Transcription >> Co-factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
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Relevance
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KDM5C / JARID1C / SMCX is expressed in a variety of tissues and it's highest levels are found in skeletal and brain extracts. Defects in SMCX are the cause of X-linked mental retardation (XLMR). Etiologically, mental retardation is a very heterogeneous condition that involves environmental, stochastic and/or genetic factors.
KDM5C specifically demethylates di and tri methyl histone H3 at K4.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KDM5C / Jarid1C / SMCX antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for KDM5C / Jarid1C / SMCX antibody images, references, products related to ab72152 and other tools.
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KDM5C / Jarid1C / SMCX antibody images:
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 Western blot - KDM5C / Jarid1C / SMCX antibody (ab72152)
All lanes : KDM5C / Jarid1C / SMCX antibody (ab72152) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size : 176 kDa
Observed band size : 200 kDa (why is the actual band size different from the predicted?)
Additional bands at : 220 kDa,300 kDa. We are unsure as to the identity of these extra bands.
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Immunoprecipitation - KDM5C / Jarid1C / SMCX antibody (ab72152)
Detection of Human KDM5C / Jarid1C / SMCX by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg/IP, 20% of IP loaded) using ab72152 at 3 µg/mg lysate for IP and at 0.1 µg/ml for subsequent WB detection. Note: bands between 55 and 71 kDa represent the IgG chains.
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Search PubMed (MEDLINE) for references to KDM5C / Jarid1C / SMCX
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KDM5C / Jarid1C / SMCX antibody - more information
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Customer reviews (feedback) regarding KDM5C / Jarid1C / SMCX antibody |
Customer FAQs regarding KDM5C / Jarid1C / SMCX antibody |
Protocols for KDM5C / Jarid1C / SMCX antibody |
Price and availability of products related to KDM5C / Jarid1C / SMCX antibody |
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KDM5C / Jarid1C / SMCX antibody - related products:
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KDM5C / Jarid1C / SMCX antibody - other tools:
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Contact Abcam with a Technical Enquiry about KDM5C / Jarid1C / SMCX antibody |
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