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| Please read the definition of 'Fast-Track antibody' |
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Immunocytochemistry/ Immunofluorescence - KDM6B / JMJD3 antibody (ab38113)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KDM6B / JMJD3 antibody (ab38113)
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This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data. |
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Product Name
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KDM6B / JMJD3 antibody
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See all KDM6B / JMJD3 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to KDM6B / JMJD3
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Note on antibody grade |
Fast-Track antibodies
- Immunogen affinity purified
- high ELISA titre values
- released early (to accelerate your research)
- 950 Abpoints to the 1st researcher
who submits conclusive data (positive or negative):
Read the Fast-Track antibody Terms of Use
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Immunogen
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Synthetic peptide of Human JMJD3. (Peptide available as ab38112.)
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Predicted to react
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Hu, Ms
(Due to sequence homology)
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Abreviews
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Application notes
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Recommended dilutions
This antibody gave a positive result in ELISA against the immunizing peptide (ab38112).
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Jumonji containing proteins
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> HOX
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Relevance
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KDM6B / JMJD3 is a 181 kDa jumonji C domain contain protein. KDM6B specifically demethylates di and tri histone H3 at K27. KDM6B plays a central role in regulation of posterior development, by regulating HOX gene expression and is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.10 - 1.00 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KDM6B / JMJD3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for KDM6B / JMJD3 antibody images, references, products related to ab38113 and other tools.
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KDM6B / JMJD3 antibody images:
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This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Please read the definition of 'Fast-Track antibody'
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 Immunocytochemistry/ Immunofluorescence - KDM6B / JMJD3 antibody (ab38113)
ICC/IF image of ab38113 stained HeLa cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab38113, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, MCF7 and HepG2 cells fixed in 100% methanol at 5ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KDM6B / JMJD3 antibody (ab38113)
IHC image of KDM6B/JMJD3 staining in human liver carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38113, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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References for KDM6B / JMJD3 antibody (ab38113)
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This product has been used in:
Barradas M et al. Histone demethylase JMJD3 contributes to epigenetic control of INK4a/ARF by oncogenic RAS. Genes Dev 23:1177-82 (2009). ICC/IF; Human. PubMed: 19451218
If you publish research using ab38113 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to KDM6B / JMJD3
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KDM6B / JMJD3 antibody - more information
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KDM6B / JMJD3 antibody - related products:
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KDM6B / JMJD3 antibody - other tools:
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