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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> HOX
Overview
Product name
Anti-KDM6B / JMJD3 antibody
See all KDM6B / JMJD3 products (3) ...
Description
Rabbit polyclonal to KDM6B / JMJD3
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Dog
Immunogen
Synthetic peptide corresponding to a region within N terminal amino acids 2 - 51 (HRAVDPPGAR AAREAFALGG LSCAGAWSSC PPHPPPRSAW LPGGRCSASI) of Human KDM6B / JMJD3 (NP_001073893).
HRAVDPPGAR AAREAFALGG LSCAGAWSSC PPHPPPRSAW LPGGRCSASI
Positive control
Human fetal lung lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - KDM6B / JMJD3 antibody (ab84200)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab84200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 181 kDa.
Target
Function
Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation.
Sequence similarities
Belongs to the UTX family.
Contains 1 JmjC domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO15054
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Histone demethylase JMJD3 antibody
- JmjC domain containing protein 3 antibody
- JmjC domain-containing protein 3 antibody
see all
Database links
- Entrez Gene: 23135 Human
- Entrez Gene: 216850 Mouse
- Entrez Gene: 363630 Rat
- Omim: 611577 Human
- SwissProt: O15054 Human
- SwissProt: Q5NCY0 Mouse
- Unigene: 223678 Human
- Unigene: 261201 Mouse
see all
Anti-KDM6B / JMJD3 antibody images:
Western blot - KDM6B / JMJD3 antibody (ab84200)
Anti-KDM6B / JMJD3 antibody (ab84200) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 181 kDa
Observed band size : >168 kDa (why is the actual band size different from the predicted?)
Gel concentration: 6-18%
References for Anti-KDM6B / JMJD3 antibody (ab84200)
ab84200 has not yet been referenced specifically in any publications.
Publishing research using ab84200? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"