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KEX2 protease protein (Active) (ab96554)

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Overview

  • Product nameKEX2 protease protein (Active)
  • Protein descriptionRecombinant fragment corresponding to amino acids 114-667 of Saccharomyces cerevisiae KEX2 protease expressed in High-5 Insect Cells; 554 amino acids, MWt 59.9kDa (UniProt P13134).
  • Uniprot accessionP13134
  • Molecular weight59.900kDa
  • Protein length554 amino acids
  • Expression hostBaculovirus infected High-5 cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesPurity is greater than 95% by SDS-PAGE gel and HPLC analyses.
  • Biological activityab96554 from High-5 insect cells contains the same specific activity and recognition sequence specificity as yeast derived KEX2. 1 milligram of recombinant KEX2 contains activity equivalent to at least 40 units of yeast derived KEX2. Cleaves at the carboxyl side of K/R-R.
  • E.C. Number3.4.21.61
  • FormLyophilised:The lyophilized protein is stable at room temperature for up to 1 month. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 5mM Calcium chloride, 10mM Sodium acetate, pH 6.0
  • Concentration information loading...
  • SequenceAPPMDSSLLP VKEAEDKLSI NDPLFERQWH LVNPSFPGSD INVLDLWYNN ITGAGVVAAI VDDGLDYENE DLKDNFCAEG SWDFNDNTNL PKPRLSDDYH GTRCAGEIAA KKGNNFCGVG VGYNAKISGI RILSGDITTE DEAASLIYGL DVNDIYSCSW GPADDGRHLQ GPSDLVKKAL VKGVTEGRDS KGAIYVFASG NGGTRGDNCN YDGYTNSIYS ITIGAIDHKD LHPPYSEGCS AVMAVTYSSG SGEYIHSSDI NGRCSNSHGG TSAAAPLAAG VYTLLLEANP NLTWRDVQYL SILSAVGLEK NADGDWRDSA MGKKYSHRYG FGKIDAHKLI EMSKTWENVN AQTWFYLPTL YVSQSTNSTE ETLESVITIS EKSLQDANFK RIEHVTVTVD IDTEIRGTTT VDLISPAGII SNLGVVRPRD VSSEGFKDWT FMSVAHWGEN GVGDWKIKVK TTENGHRIDF HSWRLKLFGE SIDSSKTETF VFGNDKEEVE PAATESTVSQ YSASSTSISI SATSTSSISI GVETSAIPQT TTASTDPDSD PNTP
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab96554 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    Functional Studies FuncS: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        KEX 2Kex2pQDS1
        SRB1VMA45
      see all
  • RelevanceKex2 in the yeast Saccharomyces cerevisiae is a transmembrane, Ca2+-dependent serine protease of the subtilisin-like pro-protein convertase (SPC) family with specificity for cleavage after paired basic amino acids. At steady state, Kex2 is predominantly localized in late Golgi compartments and initiates the proteolytic maturation of pro-protein precursors that transit the distal secretory pathway. However, Kex2 localization is not static, and its itinerary apparently involves transiting out of the late Golgi and cycling back from post-Golgi endosomal compartments during its lifetime.
  • Cellular localizationGolgi apparatus; trans-Golgi network; trans-Golgi network membrane; single-pass type I membrane protein.

    • References for KEX2 protease protein (Active) (ab96554)

    ab96554 has not yet been referenced specifically in any publications.

    Publishing research using ab96554 ? Please let us know so that we can cite the reference in this datasheet

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    I have recently procured kex2 protease from abcam. I wanted to confirm about the storage buffer as mentioned in the data sheet. It mentions that the constituents are 5mM Calcium chloride and 10mM Sodium acetate at pH 6.0.

    However, I could not f...

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    Thank you for your enquiry.

    The KEX2 protease protein (ab96554)is formulated using 10 mM sodiumacetate, pH6.0 +5 mMcalcium chloride and then lyophilised from this buffer. This is the product you receive. The sodium acetate and calcium chloride ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"