Anti-KIAA0652/ATG13 antibody (ab105392)
Key features and details
- Rabbit polyclonal to KIAA0652/ATG13
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-KIAA0652/ATG13 antibody
See all KIAA0652/ATG13 primary antibodies -
Description
Rabbit polyclonal to KIAA0652/ATG13 -
Host species
Rabbit -
Specificity
ab105392 is predicted to not cross-react with other ATG members. Two isoforms of KIAA0652/ATG13 are known to exist; this antibody will recognize both isoforms.
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Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human KIAA0652/ATG13 (C terminal).
Database link: NP_001136145 -
Positive control
- WB: Rat heart tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 57 kDa.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 57 kDa. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Autophagy factor required for autophagosome formation. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. -
Sequence similarities
Belongs to the ATG13 metazoan family. -
Post-translational
modificationsPhosphorylated by ULK1 and ULK2. Phosphorylation status depends on nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. -
Cellular localization
Cytoplasm > cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane and the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation. - Information by UniProt
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Database links
- Entrez Gene: 9776 Human
- Entrez Gene: 51897 Mouse
- Entrez Gene: 362164 Rat
- SwissProt: O75143 Human
- SwissProt: Q91YI1 Mouse
- Unigene: 127403 Human
- Unigene: 28492 Mouse
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Alternative names
- ATG13 antibody
- ATG13 autophagy related 13 homolog (S. cerevisiae) antibody
- ATG13_HUMAN antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab105392 has been referenced in 7 publications.
- Shi Y et al. CircHADHA-augmented autophagy suppresses tumor growth of colon cancer by regulating autophagy-related gene via miR-361. Front Oncol 12:937209 (2022). PubMed: 36313671
- Wang S et al. miR-654-5p inhibits autophagy by targeting ATG7 via mTOR signaling in intervertebral disc degeneration. Mol Med Rep 23:N/A (2021). PubMed: 33846806
- Stavoe AK et al. Expression of WIPI2B counteracts age-related decline in autophagosome biogenesis in neurons. Elife 8:N/A (2019). PubMed: 31309927
- Zhou M et al. Shen-Yuan-Dan Capsule Attenuates Atherosclerosis and Foam Cell Formation by Enhancing Autophagy and Inhibiting the PI3K/Akt/mTORC1 Signaling Pathway. Front Pharmacol 10:603 (2019). PubMed: 31214032
- Shin JH et al. Down-regulated TMED10 in Alzheimer disease induces autophagy via ATG4B activation. Autophagy 15:1495-1505 (2019). PubMed: 30821607
- Liu W et al. Electroacupuncture protects against ischemic stroke by reducing autophagosome formation and inhibiting autophagy through the mTORC1-ULK1 complex-Beclin1 pathway. Int J Mol Med 37:309-18 (2016). WB . PubMed: 26647915
- Huang J et al. HMGB1 promotes drug resistance in osteosarcoma. Cancer Res 72:230-8 (2012). WB ; Human . PubMed: 22102692