Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Kinesin-like
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All lanes : Anti-KIF13A antibody (ab72414) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size : 203 kDa
Observed band size : 238 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutes
Detection of KIF13A by Western Blot of Immunprecipitate.
ab72414, at 1µg/ml, staining KIF13A in HeLa whole cell lysate immunoprecipitated using ab72414 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
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