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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Kinesin-like
Overview
Product name
Anti-KIF13A antibody
See all KIF13A products (2) ...
Description
Rabbit polyclonal to KIF13A
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to a region between residues 1675 and 1725 of human KIF13A (NP_071396.3)
Positive control
HeLa whole cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab72414 was affinity purified using an epitope specific to KIF13A immobilized on solid support.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Kinesin-like
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000.Detects a band of approximately 238 kDa (predicted molecular weight: 203 kDa).
IP: Use at 2-5 µg/mg of lysate.
Target
Relevance
KIF13A belongs to the kinesin like protein family. It is a plus end directed microtubule dependent motor protein involved in mannose-6-phosphate receptor (M6PR) transport to the plasma membrane.
Alternative names
- Kinesin family member 13A antibody
- bA500C11.2 antibody
- FLJ27232 antibody
see all
Database links
- Entrez Gene: 63971 Human
- Omim: 605433 Human
- SwissProt: Q9H1H9 Human
Anti-KIF13A antibody images:
Western blot - KIF13A antibody (ab72414)
All lanes : Anti-KIF13A antibody (ab72414) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size : 203 kDa
Observed band size : 238 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutes
Immunoprecipitation - KIF13A antibody (ab72414)
Detection of KIF13A by Western Blot of Immunprecipitate.
ab72414, at 1µg/ml, staining KIF13A in HeLa whole cell lysate immunoprecipitated using ab72414 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
References for Anti-KIF13A antibody (ab72414)
ab72414 has not yet been referenced specifically in any publications.
Publishing research using ab72414? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"