Overview

  • Product name
  • Description
    Rabbit polyclonal to KIF13B
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 108-157 (GKSYTMMGTA DQPGLIPRLC SGLFERTQKE ENEEQSFKVE VSYMEIYNEK) of Human KIF13B (NP_056069).

  • Positive control
    • HepG2 cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 203 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    May be involved in reorganization of the cortical cytoskeleton. May be functionally important for the intracellular trafficking of MAGUKs and associated protein complexes.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the kinesin-like protein family.
    Contains 1 CAP-Gly domain.
    Contains 1 FHA domain.
    Contains 1 kinesin-motor domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • GAKIN antibody
    • Guanylate kinase associated kinesin antibody
    • KI13B_HUMAN antibody
    • KIAA0639 antibody
    • KIF 13B antibody
    • KIF13B antibody
    • Kinesin 13B antibody
    • Kinesin family member 13B antibody
    • Kinesin like protein GAKIN antibody
    • Kinesin like protein KIF13B antibody
    • Kinesin-like protein GAKIN antibody
    • Kinesin-like protein KIF13B antibody
    • OTTHUMP00000225649 antibody
    see all

Images

  • IHC image of KIF13B staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86528, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-KIF13B antibody (ab86528) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 203 kDa
    Observed band size : 203 kDa
    Additional bands at : 38 kDa,65 kDa. We are unsure as to the identity of these extra bands.Gel concentration 6-18%
  • Anti-KIF13B antibody (ab86528) at 1 µg/ml (in blocking buffer for 16 hours at 4°C) + Whole cell lysate of human AGS Gastric carcinoma cells at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 203 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% BSA for 1 hour at 23°C

    See Abreview

References

ab86528 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 Mouse Fibroblasts)
Loading amount
10 µg
Specification
NIH 3T3 Mouse Fibroblasts
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Sep 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (AGS Gastric Carcinoma cells)
Loading amount
20 µg
Specification
AGS Gastric Carcinoma cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jun 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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