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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab3746? Please let us know so that we can cite the reference in this datasheet
ab3746 has been referenced in 1 publications.
- Carleton M et al. RNA interference-mediated silencing of mitotic kinesin KIF14 disrupts cell cycle progression and induces cytokinesis failure. Mol Cell Biol 26:3853-63 (2006). ICC/IF; Human. PubMed: 16648480
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunoprecipitation - KIF14 antibody (ab3746)
Sample: Hela whole cell lysate
1) WB - 50
2) IP - 1mg.
Antibody: Affinity purified Rabbit anti-KIF (ab3746)
1) & 2) WB - 1 / 4000 dilution.
2) IP - concentration of 3
Detection: ECL.
Immunocytochemistry/ Immunofluorescence-KIF14 antibody(ab3746)
ICC/IF image of ab3746 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3746, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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