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Immunoprecipitation - KIF14 antibody (ab3746)
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Product Name
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KIF14 antibody
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See all KIF14 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to KIF14
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Immunogen
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Synthetic peptide (Human) - which represented a portion of human Kinesin Family Member 14 (KIF 14)encoded within exon 2.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution. PubMed 16648480.
IP: Use at a concentration of 1 - 4 µg/ml.
WB: 1/2000 - 1/10000. Predicted molecular weight: 190 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa whole cell extract.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Kinesin-like
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Kinesin
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Microtubules
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Relevance
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KIF14 belongs to the kinesin-like protein family. It contains one FHA domain and one kinesin-motor domain. KIF14 plays an essential role in cytokinesis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KIF14 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for KIF14 antibody images, references, products related to ab3746 and other tools.
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KIF14 antibody images:
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 Immunoprecipitation - KIF14 antibody (ab3746)
Sample: Hela whole cell lysate
1) WB - 50µg.
2) IP - 1mg.
Antibody: Affinity purified Rabbit anti-KIF (ab3746)
1) & 2) WB - 1 / 4000 dilution.
2) IP - concentration of 3µg / mg of lysate.
Detection: ECL.
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References for KIF14 antibody (ab3746)
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This product has been used in:
Carleton M et al. RNA interference-mediated silencing of mitotic kinesin KIF14 disrupts cell cycle progression and induces cytokinesis failure. Mol Cell Biol 26:3853-63 (2006). ICC/IF; Human. PubMed: 16648480
If you publish research using ab3746 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to KIF14
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KIF14 antibody - more information
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Customer reviews (feedback) regarding KIF14 antibody |
Customer FAQs regarding KIF14 antibody |
Protocols for KIF14 antibody |
Price and availability of products related to KIF14 antibody |
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KIF14 antibody - related products:
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KIF14 antibody - other tools:
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Contact Abcam with a Technical Enquiry about KIF14 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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