Anti-KIF14 antibody (ab3746)

  Immunoprecipitation - KIF14 antibody (ab3746)

Sample: Hela whole cell lysate

1) WB - 50µg.

2) IP - 1mg.

Antibody: Affinity purified Rabbit anti-KIF (ab3746)

1) & 2) WB - 1 / 4000 dilution.

2) IP - concentration of 3µg / mg of lysate.

Detection: ECL.

  Immunocytochemistry/ Immunofluorescence-KIF14 antibody(ab3746)

ICC/IF image of ab3746 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3746, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.