Overview

  • Product nameAnti-KIF14 antibodySee all KIF14 primary antibodies ...
  • Description
    Rabbit polyclonal to KIF14
  • Tested applicationsWB, IP more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1600 and the C-terminus (residue 1648) of human KIF14 (NP_055690.1).

  • Positive control
    • Whole cell lysates from Hela cells and 293T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab71155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/2000 - 1/10000. Detects a band of approximately 210 kDa (predicted molecular weight: 186 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionPlays an essential role in cytokinesis.
  • Sequence similaritiesBelongs to the kinesin-like protein family.
    Contains 1 FHA domain.
    Contains 1 kinesin-motor domain.
  • Developmental stageUp-regulated in cells progressing through G2/M phase.
  • Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Nuclear localization observed during interphase in PubMed:16431929 or triggered by entry into mitosis in PubMed:16648480. Cytoplasmic in interphase (PubMed:16648480) and metaphase cells (PubMed:16431929). From prophase to metaphase, accumulates at the developing spindle poles and their associated microtubules. During anaphase, accumulates at the spindle midzone. Localization to the central spindle and midbody during anaphase is dependent upon PRC1 and CIT presence. In cells ready to undergo abscission, concentrates at the contractile ring.
  • Target information above from: UniProt accession Q15058 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • KIAA0042 antibody
    • KIF 14 antibody
    • KIF14 antibody
    • KIF14_HUMAN antibody
    • Kinesin Family Member 14 antibody
    • Kinesin like protein KIF14 antibody
    • Kinesin-like protein KIF14 antibody
    • MGC142302 antibody
    see all

Anti-KIF14 antibody images

  • All lanes : Anti-KIF14 antibody (ab71155) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 186 kDa
    Observed band size : 210 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 180 kDa,190 kDa,60 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  • Immunoprecipitation of HeLa whole cell lysate (1 mg) using ab71155 at 3 µg/mg lysate (lane 1) or control IgG (lane 2). 20% of immunoiprecipitate was analysed by Western blot using ab71155 at 1 µg/ml.

References for Anti-KIF14 antibody (ab71155)

ab71155 has not yet been referenced specifically in any publications.

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