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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Kinesin
Overview
Product name
Anti-KIF3A antibody
See all KIF3A products (6) ...
Description
Rabbit polyclonal to KIF3A
Tested applications
WB, IF, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide conjugated to KLH, corresponding to amino acids 682-701 of Mouse KIF3A. This sequence is identical in human.
Positive control
Rat brain homogenate, PC12 cell line.
General notes
Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Wnt >> Surface Molecules
Neuroscience >> Neurotransmitter >> Transporters >> Other Transporters
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Kinesin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000Predicted molecular weight: 85 kDa.(This concentration is determined by blotting using a rat brain homogenate and a whole cell extract of rat pheochromocytoma PC12 cell line. Staining of KIF3A is specifically inhibited by the KIF3A immunizing peptide. )
IF: 1/100(This concentration is determined by Indirect Immunofluorescence using frozen sections of rat cerebellum.)
IHC-P: Use at an assay dependent dilution. (PubMed: 20421870)
Target
Function
Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro.
Sequence similarities
Belongs to the kinesin-like protein family. Kinesin II subfamily.
Contains 1 kinesin-motor domain.
Cellular localization
Cytoplasm > cytoskeleton.
Target information above from: UniProt accessionQ9Y496
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- KIF 3 antibody
- KIF 3A antibody
- KIF3 antibody
see all
Database links
- Entrez Gene: 11127 Human
- Entrez Gene: 16568 Mouse
- Entrez Gene: 84392 Rat
- Omim: 604683 Human
- SwissProt: Q9Y496 Human
- SwissProt: P28741 Mouse
- Unigene: 43670 Human
- Unigene: 88694 Mouse
Anti-KIF3A antibody images:
Western blot - KIF3A antibody (ab11259)
Predicted band size : 85 kDa
ab11259 (1/2000) detecting KIF3A in Human Hela Cells. As a control, cells were transfected with scramble siRNA (left) and KIF3A siRNA (right). Please refer to abreview for further experimental details.
This image is courtesy of an anonymous Abreview
Immunocytochemistry/ Immunofluorescence - Anti-KIF3A antibody (ab11259)
ab11259 staining KIF3A in murine primary hippocampal neurons by Immunocytochemistry/ Immunofluorescence.Cells were fixed with methanol, blocked with 1% BSA for 1 hour at 22°C and then incubated with ab11259 at a 1/50 dilution for 2 hours at 22°C. The secondary used was a Cy3-conjugated donkey anti-rabbit polyclonal, used at a 1/500 dilution.
Image courtesy of an anonymous Abreview.
References for Anti-KIF3A antibody (ab11259)
This product has been referenced in:
- Massinen Set al. Increased Expression of the Dyslexia Candidate Gene DCDC2 Affects Length and Signaling of Primary Cilia in Neurons. PLoS One 6:e20580 (2011). WB; Rat.Read more (PubMed: 21698230) »
- Evans RJet al. The retinitis pigmentosa protein RP2 links pericentriolar vesicle transport between the Golgi and the primary cilium. Hum Mol Genet 19:1358-67 (2010). ICC/IF; Human.Read more (PubMed: 20106869) »
See all 9 publications for this product
Publishing research using ab11259? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"