The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Predicted molecular weight: 85 kDa. This concentration is determined by blotting using a rat brain homogenate and a whole cell extract of rat pheochromocytoma PC12 cell line. Staining of KIF3A is specifically inhibited by the KIF3A immunizing peptide.
Use at an assay dependent concentration.
Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro.
Belongs to the kinesin-like protein family. Kinesin II subfamily. Contains 1 kinesin-motor domain.
Microtubule plus end directed kinesin motor 3A antibody
Microtubule plus end-directed kinesin motor 3A antibody
Western blot - Anti-KIF3A antibody (ab11259)This image is courtesy of an anonymous Abreview
Predicted band size : 85 kDa
This image is courtesy of an anonymous Abreview
ab11259 (1/2000) detecting KIF3A in Human Hela Cells. As a control, cells were transfected with scramble siRNA (left) and KIF3A siRNA (right). Please refer to abreview for further experimental details.
Immunocytochemistry/ Immunofluorescence - Anti-KIF3A antibody (ab11259)Image courtesy of an anonymous Abreview.
ab11259 staining KIF3A in murine primary hippocampal neurons by Immunocytochemistry/ Immunofluorescence. Cells were fixed with methanol, blocked with 1% BSA for 1 hour at 22°C and then incubated with ab11259 at a 1/50 dilution for 2 hours at 22°C. The secondary used was a Cy3-conjugated donkey anti-rabbit polyclonal, used at a 1/500 dilution.
Hoang-Minh LB et al. PCM1 Depletion Inhibits Glioblastoma Cell Ciliogenesis and Increases Cell Death and Sensitivity to Temozolomide. Transl Oncol9:392-402 (2016).
Read more (PubMed: 27661404) »
Schueler M et al. DCDC2 mutations cause a renal-hepatic ciliopathy by disrupting Wnt signaling. Am J Hum Genet96:81-92 (2015).
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