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Overview

  • Product nameKIM1 Mouse ELISA KitSee all KIM1 kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeSandwich
  • Sensitivity
    < 2 pg/ml
  • Range
    31.2 pg/ml - 2000 pg/ml
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    ab119596 KIM1 Mouse ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse KIM1 specific-specific polyclonal antibodies were precoated onto 96-well plates. The Mouse specific detection monoclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse KIM1 amount of sample captured in plate.

  • Notes

    Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionMay play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
  • Tissue specificityWidely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. TIM family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationMembrane.
  • Target information above from: UniProt accession Q96D42 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        HAVCRHAVcr-1Havcr1
        HAVR1_HUMANHepatitis A virus cellular receptor 1Kidney injury molecule 1KIM-1T cell immunoglobin domain and mucin domain protein 1T-cell immunoglobulin and mucin domain-containing protein 1T-cell membrane protein 1TIMTIM-1TIM1TIMD-1TIMD1
      see all
  • Database links
  • Applications

    Our Abpromise guarantee covers the use of ab119596 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Sandwich ELISA sELISA

    KIM1 Mouse ELISA Kit images

    • Representative standard curve using ab119596.

    Protocols

    References for KIM1 Mouse ELISA Kit (ab119596)

    ab119596 has not yet been referenced specifically in any publications.

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