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Overview

  • Product nameKIM1 Rat ELISA KitSee all KIM1 kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeSandwich
  • Sensitivity
    < 2 pg/ml
  • Range
    31.2 pg/ml - 2000 pg/ml
  • Species reactivity
    Reacts with: Rat
  • Product overview

    ab119597 KIM1 Rat ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat KIM1 specific-specific polyclonal antibodies were precoated onto 96-well plates. The Rat specific detection monoclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat KIM1 amount of sample captured in plate.

     

    No detectable cross-reactivity with any other cytokine.

  • Notes

    Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionMay play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair.
  • Tissue specificityWidely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. TIM family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession Q96D42 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        HAVCRHAVcr-1Havcr1
        HAVR1_HUMANHepatitis A virus cellular receptor 1Kidney injury molecule 1KIM-1T cell immunoglobin domain and mucin domain protein 1T-cell immunoglobulin and mucin domain-containing protein 1T-cell membrane protein 1TIMTIM-1TIM1TIMD-1TIMD1
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119597 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      KIM1 Rat ELISA Kit images

      • Sandwich ELISA - KIM1 Rat ELISA Kit (ab119597)
        Sandwich ELISA - KIM1 Rat ELISA Kit (ab119597)
        Representative standard curve using ab119597.

      Protocols

      References for KIM1 Rat ELISA Kit (ab119597)

      ab119597 has not yet been referenced specifically in any publications.

      Publishing research using ab119597 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab119597 Submit a Question for ab119597

      Displaying 1 - 5 of 5 results for Abreviews and Q&A

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      Votes: Highest First

      I've been using this kit with rat urine. Is this kit pH dependent? Should we be adjusting the pH of our samples to ensure accuracy?

      The pH of rat urine is close to 7.0, and there is no need to adjust the pH before use with the kit.

      I found you have the Rat Kim-1 ELISA kit. Do you have the Rat Kim-1 antibody for immuohistochemistry?

      Thank you for contacting us.
      We have several TIM1 antibodies (TIM1 is a synonym for KIM1).
      Two of these antibodies were tested in IHC with paraffin sections with human and mouse, but not rat.
      If you like to try using these antibodies in rat...

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      As I’m not familiar with these items, I tried to find the ab codes through your site.
      For the items I found a relative code I have update the list.
      For those I don’t have a reference code, this is because either no products matches or ...

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      I have received information regarding

      ab119589 Cystatin C Human ELISA Kit
      ab119590 Cystatin C Mouse ELISA Kit
      ab119596 KIM1 Mouse ELISA Kit
      ab119597 KIM1 Rat ELISA Kit
      ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit
      ab119601 Lipoc...

      Read More

      As I’m not familiar with these items, I tried to find the ab codes through your site.
      For the items I found a relative code I have update the list.
      For those I don’t have a reference code, this is because either no products matches or ...

      Read More

      Thank you for contacting us.


      To let you know, we are currently investigating the Porcine (pig) species, cross-reactivity information for following kits ab119602, ab113326, ab119600, ab119601, ab119590 and ab119589. We will send you the detai...

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      Thank you for your prompt reply. This is the information I needed. I am also looking to purchase a similar style ELISA kit for rat kidney injury molecule-1 (Rat KIM-1). Does your company sell that item?

      Thank you for contacting us. It is my pleasure to help.

      We do offer a KIM1 rat ELISA kit as well. This kit has a similar system containing one plate made of twelve 8 well strips. The product number is ab119597 and may be found at the followin...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"