Mouse KIM1 ELISA Kit (TIM-1) (ab119596)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 31.2 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse KIM1 ELISA Kit (TIM-1)
See all TIM 1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 87pg/ml 6.87 = 7.9% 2 16 279pg/ml 14.78 = 5.3% 3 16 842pg/ml 36.2 = 4.3% Inter-assay Sample n Mean SD CV% 1 24 85pg/ml 7.31 = 8.6% 2 24 261pg/ml 14.87 = 5.7% 3 24 792pg/ml 38.8 = 4.9% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 2 pg/ml -
Range
31.2 pg/ml - 2000 pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s mouse KIM-1 (TIM-1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse KIM-1 (TIM-1) in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
A KIM-1 (TIM-1) specific goat polyclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and are incubated. A biotinylated detection polyclonal antibody from goat specific for KIM-1 (TIM-1) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse KIM-1 (TIM-1) amount of sample captured in plate.
Get higher sensitivity in only 90 minutes with Mouse KIM-1 ELISA Kit (TIM1) (ab213477) from our SimpleStep ELISA® range.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-mouse KIM1 antibody Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti-Mouse KIM1 antibody 1 x 100µl 1 x 100µl Lyophilized recombinant Mouse KIM1 standard 2 x 10ng 2 x 10ng Plate Seal 1 x 4 units 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
Research areas
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Function
May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair. -
Tissue specificity
Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses. -
Sequence similarities
Belongs to the immunoglobulin superfamily. TIM family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD365
- HAVCR
- HAVCR 1
see all -
Database links
- Entrez Gene: 171283 Mouse
- SwissProt: Q5QNS5 Mouse
Associated products
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SimpleStep ELISA kits
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab119596 has been referenced in 10 publications.
- Nguyen LT et al. Blood DNA Methylation Predicts Diabetic Kidney Disease Progression in High Fat Diet-Fed Mice. Nutrients 14:N/A (2022). PubMed: 35215435
- Al-Bataineh MM et al. KIM-1-mediated anti-inflammatory activity is preserved by MUC1 induction in the proximal tubule during ischemia-reperfusion injury. Am J Physiol Renal Physiol 321:F135-F148 (2021). PubMed: 34151589
- Jiménez-Castilla L et al. Nephroprotective Effects of Synthetic Flavonoid Hidrosmin in Experimental Diabetic Nephropathy. Antioxidants (Basel) 10:N/A (2021). PubMed: 34943023
- Gibbs KM et al. Effects of General Anesthesia on 2 Urinary Biomarkers of Kidney Injury-Hepatitis A Virus Cellular Receptor 1 and Lipocalin 2-in Male C57BL/6J Mice. J Am Assoc Lab Anim Sci 58:21-29 (2019). PubMed: 30538007
- Papu John AS et al. Hydrogen Sulfide Inhibits Ca2+-induced Mitochondrial Permeability Transition Pore (MPTP) Opening in Type-1 Diabetes. Am J Physiol Endocrinol Metab N/A:N/A (2019). PubMed: 31039005
- Yang X et al. Precision toxicology shows that troxerutin alleviates ochratoxin A-induced renal lipotoxicity. FASEB J 33:2212-2227 (2019). PubMed: 30247986
- Zahr RS et al. Renal protection by atorvastatin in a murine model of sickle cell nephropathy. Br J Haematol 181:111-121 (2018). PubMed: 29527679
- Song MF et al. Sema 3A as a biomarker of the activated mTOR pathway during hexavalent chromium-induced acute kidney injury. Toxicol Lett 299:226-235 (2018). PubMed: 30236763
- Jackson EK et al. Renal 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase Is an Important Determinant of AKI Severity after Ischemia-Reperfusion. J Am Soc Nephrol 27:2069-81 (2016). PubMed: 26574047
- Wang Y et al. Cinnabar induces renal inflammation and fibrogenesis in rats. Biomed Res Int 2015:280958 (2015). Rat . PubMed: 25734058