Recombinant Anti-KLC3 antibody [EPR11149(2)] (ab180523)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11149(2)] to KLC3
- Suitable for: ICC/IF, Flow Cyt (Intra), WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-KLC3 antibody [EPR11149(2)]
See all KLC3 primary antibodies -
Description
Rabbit monoclonal [EPR11149(2)] to KLC3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt (Intra), WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human KLC3 aa 350-450. The exact sequence is proprietary.
Database link: Q6P597 -
Positive control
- Jurkat, U87-MG, HepG2 and BxPC-3 cell lysates; Human liver tissue; HepG2 and Jurkat cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11149(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/1000.
|
|
Flow Cyt (Intra) |
1/10.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/10000 - 1/50000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
|
|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
IP |
1/40.
|
Notes |
---|
ICC/IF
1/1000. |
Flow Cyt (Intra)
1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/40. |
Target
-
Function
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. -
Sequence similarities
Belongs to the kinesin light chain family.
Contains 5 TPR repeats. -
Domain
The heptad repeat (HR) motif is sufficient for interaction with kinesin heavy (KHL) chains and ODF1. The TPR region is involved in mitochondrial binding. -
Cellular localization
Cytoplasm > cytoskeleton. Elongating spermatid tail midpiece, localized in outer dense fibers (ODFs) and associates with mitochondria. - Information by UniProt
-
Database links
- Entrez Gene: 147700 Human
- Entrez Gene: 232943 Mouse
- Omim: 601334 Human
- SwissProt: Q6P597 Human
- SwissProt: Q91W40 Mouse
- Unigene: 298079 Human
- Unigene: 213403 Mouse
-
Alternative names
- Kinesin light chain 2 antibody
- Kinesin light chain 3 antibody
- KLC2 antibody
see all
Images
-
All lanes : Anti-KLC3 antibody [EPR11149(2)] (ab180523) at 1/50000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : U-87 MG cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : BxPC-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDa -
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling KLC3 with ab180523 at 1/250 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG secondary and counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling KLC3 with ab180523 at 1/1000 dilution followed by Goat anti-rabbit IgG (Dylight 555) at 1/250 dilution and counterstained with DAPI.
-
Western blot analysis of immunoprecipitation pellet from BxPC-3 cell lysate immunoprecipitated using ab180523 at 1/40 dilution (lane 1). Negative control (lane 2). Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1500 dilution was used as secondary.
-
Intracellular flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed)labeling KLC3 with ab180523 at 1/10 dilution. Goat anti-rabbit IgG (FITC)at 1/75 dilution was used as the secondary antibody.Rabbit monoclonal IgG was used as the isotype control.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (3)
ab180523 has been referenced in 3 publications.
- Chung J et al. Super-resolution imaging of platelet-activation process and its quantitative analysis. Sci Rep 11:10511 (2021). PubMed: 34006947
- Go S et al. Super-resolution imaging reveals cytoskeleton-dependent organelle rearrangement within platelets at intermediate stages of maturation. Structure 29:810-822.e3 (2021). PubMed: 34143977
- Georgiades P et al. The flexibility and dynamics of the tubules in the endoplasmic reticulum. Sci Rep 7:16474 (2017). PubMed: 29184084