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- Secondary antibodies
- Lysates
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Product name
Anti-KLC4 antibody
See all KLC4 products (3) ...
Description
Mouse polyclonal to KLC4
Tested applications
Cross reactivity
Reacts with
Rat, Human
Immunogen
Full length human KLC4 protein (NP_958929.1).
Positive control
Rat brain tissue lysate, HeLa cell lysate and KLC4 transfected 293T cell line lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Kinesin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab89040 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 69 kDa.
Target
Function
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.
Sequence similarities
Belongs to the kinesin light chain family.
Contains 7 TPR repeats.
Cellular localization
Cytoplasm > cytoskeleton.
Target information above from: UniProt accessionQ9NSK0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Kinesin light chain 4 antibody
- kinesin like 8 antibody
- Kinesin like protein 8 antibody
see all
Database links
- Entrez Gene: 89953 Human
- Entrez Gene: 316226 Rat
- SwissProt: Q9NSK0 Human
- SwissProt: Q5PQM2 Rat
- Unigene: 655123 Human
- Unigene: 16800 Rat
Anti-KLC4 antibody images:
Western blot - KLC4 antibody (ab89040)
Anti-KLC4 antibody (ab89040) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size : 69 kDa
Observed band size : 69 kDa
Western blot - KLC4 antibody (ab89040)
Anti-KLC4 antibody (ab89040) at 1 µg/ml + Rat brain tissue lysate at 50 µg
Predicted band size : 69 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Western blot - KLC4 antibody (ab89040)
Anti-KLC4 antibody (ab89040) at 1 µg/ml + Rat brain tissue lysate at 50 µg
Predicted band size : 69 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Western blot - KLC4 antibody (ab89040)
All lanes : Anti-KLC4 antibody (ab89040) at 1 µg/ml
Lane 1 : KLC4 transfected 293T cell line lysate
Lane 2 : Non-transfected 293T cell line lysate
Lysates/proteins at 25 µg per lane.
Predicted band size : 69 kDa
Observed band size : 69 kDa
References for Anti-KLC4 antibody (ab89040)
ab89040 has not yet been referenced specifically in any publications.
Publishing research using ab89040? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"