Overview

  • Product nameAnti-KLF15 antibodySee all KLF15 primary antibodies ...
  • Description
    Goat polyclonal to KLF15
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig
  • Immunogen

    Synthetic peptide: VDHLLPVDENFSS, corresponding to N terminal amino acids 2-14 of Human KLF15.

  • Positive control
    • Human Liver or HepG2 lysate
  • General notes

    Principal Names – KLF15; Kruppel-like factor 15; KKLF; KKLF protein; kidney-enriched Kruppel-like factor Official Gene Symbol - KLF15 GenBank Accession Number – NP_054798 LocusLink ID - 28999 (human); 66277 (mouse); 85497 (rat)

     

    We no longer guarantee this product for mouse because of recent complaints. The immunogen however shares 92% sequence homology with mouse so we still predict the antibody will cross-react. We will welcome any feedback from customers.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab2647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).Can be blocked with Human KLF15 peptide (ab22927).

Approx 48kDa band observed in lysates of hepatoblastoma cell line HepG2 (calculated MW of 44.0kDa according to NP_054798.1). Older batches detected a band of approximately 65 kDa in Human Liver lysate(predicted molecular weight: 46 kDa). Note the higher than expected size is in agreement with previous Western results (Mol Cell Biol 2000 Oct;20(19):7319-31).

Target

Anti-KLF15 antibody images



  • Predicted band size : 44 kDa


    ab2647 staining (1 µg/ml) of Mouse skeletal muscle lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.

References for Anti-KLF15 antibody (ab2647)

This product has been referenced in:
  • Mallipattu SK  et al. Kruppel-like Factor 15 (KLF15) Is a Key Regulator of Podocyte Differentiation. J Biol Chem 287:19122-35 (2012). WB ; Mouse . Read more (PubMed: 22493483) »
  • Teylaert B  et al. Molecular cloning, characterization, genomic organization and promoter analysis of the a1,6-fucosyltransferase gene (fut8) expressed in the rat hybridoma cell line YB2/0. BMC Biotechnol 11:1 (2011). WB, EMSA ; Rat . Read more (PubMed: 21208406) »

See all 3 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab2647.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"