Anti-KLF4 antibody (ab216875)
Key features and details
- Rabbit polyclonal to KLF4
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-KLF4 antibody
See all KLF4 primary antibodies -
Description
Rabbit polyclonal to KLF4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: U-87, C2C12, THP-1, J774A.1, PC-3, HT-29, A549 and HeLa whole cell lysates. ICC/IF: PC-3 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa).
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa). |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
Domain
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9314 Human
- Entrez Gene: 16600 Mouse
- Omim: 602253 Human
- SwissProt: O43474 Human
- SwissProt: Q60793 Mouse
- Unigene: 376206 Human
- Unigene: 4325 Mouse
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Alternative names
- Endothelial Kruppel like zinc finger protein antibody
- Epithelial zinc finger protein EZF antibody
- EZF antibody
see all
Images
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Immunofluorescent analysis of 0.5% PFA-fixed (A and C) and 0.5% methanol-fixed (B and D) PC-3 (human prostate adenocarcinoma cell line) cells labeling KLF4 with ab216875 at 10 µg/ml (green).
The nuclear counter stain is DAPI (blue).
Secondary antibody only control: C and D.
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All lanes : Anti-KLF4 antibody (ab216875) at 1/1000 dilution
Lanes 1 & 13 : MW ladder (opal pre-stained)
Lane 2 : U-87 whole cell lysate
Lane 3 : C2C12 whole cell lysate
Lane 4 : NIH/3T3 whole cell lysate
Lane 5 : THP-1 whole cell lysate
Lane 6 : J774A.1 whole cell lysate
Lane 7 : PC-3 whole cell lysate
Lane 8 : HT-29 whole cell lysate
Lane 9 : Mouse colon lysate
Lane 10 : A549 whole cell lysate
Lane 11 : MOLT-4 whole cell lysate
Lane 12 : HeLa whole cell lysate
Secondary
All lanes : Rabbit secondary HRP antibody at 1/70000 dilution
Developed using the ECL technique.
Predicted band size: 54 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking buffer: Blotto 5%.
Dilution buffer: 1X PBS, 0.01% azide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab216875 has been referenced in 1 publication.
- Isono W et al. The combination of dibenzazepine and a DOT1L inhibitor enables a stable maintenance of human naïve-state pluripotency in non-hypoxic conditions. Regen Ther 15:161-168 (2020). PubMed: 33426214