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Western blot - KLF4 antibody (ab26648)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KLF4 antibody (ab26648)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KLF4 antibody (ab26648)
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Product Name
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KLF4 antibody
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See all KLF4 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to KLF4
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human KLF4.
(Peptide available as ab28257.)
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Reacts with
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Hu
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Predicted to react
(species key)
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Dog
(Due to sequence homology)
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a dilution of 1/2000 for 1 hr.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Krueppel like factor
Chromatin and Nuclear Signaling >> Transcription >> Krueppel like factor
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Differentiation
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Other
Stem Cells >> Embryonic Stem Cells >> Intracellular
Chromatin and Nuclear Signaling >> Transcription >> Other factors
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Relevance
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KLF4 is a transcription factor that works with Sp1 to activate the Laminin gamma1 chain gene. It binds the CACCC core sequence. KLF4 may be involved in the differentiation of epithelial cells and may also function in the development of the skeleton and kidney. KLF4 also has roles as both an oncogene and a tumor suppressor.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.10 - 0.70 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KLF4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for KLF4 antibody images, references, products related to ab26648 and other tools.
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KLF4 antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KLF4 antibody (ab26648)
ab26648 staining KLF4 in human spleen tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in citrate buffer pH6.0 and blocking for 15 minutes at 20°C (5 minutes for peroxidase block and 10 minutes for protein block). The primary antibody was diluted 1/2000 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used undiluted as secondary. This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KLF4 antibody (ab26648)
ab26648 staining KLF4 in mouse brain tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in citrate buffer pH6.0 and blocking for 15 minutes at 20°C (5 minutes for peroxidase block and 10 minutes for protein block). The primary antibody was diluted 1/2000 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used undiluted as secondary. This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
See Abreview
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References for KLF4 antibody (ab26648)
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This product has been used in: (two most recent references)
Ram
rez-Valle F et al. eIF4GI links nutrient sensing by mTOR to cell proliferation and inhibition of autophagy. J Cell Biol 181:293-307 (2008). WB; Human. PubMed: 18426977
Kang BY et al. Differential gene expression profiling in HELLP syndrome placentas. Reprod Sci 15:285-94 (2008). PubMed: 18421023
If you publish research using ab26648 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to KLF4
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KLF4 antibody - more information
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Customer reviews (feedback) regarding KLF4 antibody |
Customer FAQs regarding KLF4 antibody |
Protocols for KLF4 antibody |
Price and availability of products related to KLF4 antibody |
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KLF4 antibody - related products:
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KLF4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about KLF4 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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