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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-KLF4 antibody
See all KLF4 products (8) ...
Description
Rabbit polyclonal to KLF4
Specificity
Some of our customers have successfully used ab26648 on mouse, please see PubMed ID: 21993235. However, we have been unable to show cross-reactivity with this species and therefore we cannot guarantee this product will consistently work on mouse. Please contact our technical support for further information.
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Rat, Dog
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human KLF4.
(Peptide available as ab27825.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Krueppel like factor
Epigenetics and Nuclear Signaling >> Transcription >> Krueppel like factor
Cardiovascular >> Heart >> Hypertrophy >> Transcription factors
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Differentiation
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Other
Stem Cells >> Embryonic Stem Cells >> Intracellular
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab26648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/2000(for 1 hour)
WB: Use a concentration of 1 - 5 µg/ml.Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
Target
Function
Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
Domain
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO43474
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Endothelial Kruppel like zinc finger protein antibody
- Epithelial zinc finger protein EZF antibody
- EZF antibody
see all
Database links
- Entrez Gene: 9314 Human
- Entrez Gene: 114505 Rat
- Omim: 602253 Human
- SwissProt: O43474 Human
- Unigene: 376206 Human
- Unigene: 7719 Rat
Anti-KLF4 antibody images:
Western blot - KLF4 antibody (ab26648)
Anti-KLF4 antibody (ab26648) at 1 µg/ml + Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate (ab3950) at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 50 kDa
Observed band size : 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KLF4 antibody (ab26648)
ab26648 staining KLF4 in human spleen tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in citrate buffer pH6.0 and blocking for 15 minutes at 20°C (5 minutes for peroxidase block and 10 minutes for protein block). The primary antibody was diluted 1/2000 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.
This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
References for Anti-KLF4 antibody (ab26648)
This product has been referenced in:
- Tavernier Get al. Activation of pluripotency-associated genes in mouse embryonic fibroblasts by non-viral transfection with in vitro-derived mRNAs encoding Oct4, Sox2, Klf4 and cMyc. Biomaterials : (2011). ICC/IF, Flow Cyt; Mouse.Read more (PubMed: 21993235) »
- Nelson TJet al. Induced pluripotent reprogramming from promiscuous human stemness related factors. Clin Transl Sci 2:118-26 (2009). WB; Human.Read more (PubMed: 20161095) »
See all 4 publications for this product
Publishing research using ab26648? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"