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Overview

  • Product nameAnti-KLHL20 antibodySee all KLHL20 primary antibodies ...
  • Description
    Rabbit polyclonal to KLHL20
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within C-terminal amino acids 481-530 (NPQENRWHTIAPMGTRRKHLGCAVYQDMIYAVGGRDDTT ELSSAERYNPR) of Human KLHL20 (NP_055273).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlHeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab108238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionSubstrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in interferon response. The BCR(KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome, thereby acting as a negative regulator of apoptosis. Also acts as a regulator of endothelial migration during angiogenesis by controlling the activation of Rho GTPases.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 6 Kelch repeats.
  • Cellular localizationCytoplasm > perinuclear region. Nucleus. Localizes in the perinuclear region in normal conditions. Following IFN-alpha or IFN-gamma treatment, it is relocalized and sequestrated to the PML nuclear bodies, preventing DAPK1 ubiquitination.

    • Target information above from: UniProt accession Q9Y2M5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Kelch like ECT2 interacting protein antibodyKelch like protein 20 antibodyKelch like protein X antibody
        Kelch motif containing protein antibodyKelch-like ECT2-interacting protein antibodyKelch-like protein 20 antibodyKelch-like protein X antibodyKHLHX antibodyKLEIP antibodyKLH20_HUMAN antibodyKLHL20 antibodyKLHLX antibodyRP3-383J4.3 antibody
      see all

    Anti-KLHL20 antibody images

    • Western blot - KLHL20 antibody (ab108238)
      Western blot - KLHL20 antibody (ab108238)
      Anti-KLHL20 antibody (ab108238) at 1 µg/ml + HeLa Cell Lysate at 10 µg

      Predicted band size : 68 kDa

    References for Anti-KLHL20 antibody (ab108238)

    ab108238 has not yet been referenced specifically in any publications.

    Publishing research using ab108238 ? Please let us know so that we can cite the reference in this datasheet

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    Western blot abreview for Anti-KLHL20 antibody

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    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (Human osteosarcoma U2OS cells)
    Loading amount 90 µg
    Specification Human osteosarcoma U2OS cells
    Gel Running Conditions Reduced Denaturing (4-18% gel)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Submitted Apr 11 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"