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Western blot - G9a antibody - ChIP Grade (ab40542)
(enlarge)
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Product Name
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KMT1C / G9a antibody - ChIP Grade
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See all KMT1C / G9a antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to KMT1C / G9a - ChIP Grade
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Immunogen
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Recombinant fusion protein, corresponding to amino acids 831-1001 of Human G9a
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Reacts with
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Hu
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Tested applications
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ChIP, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/2000 - 1/2500. Predicted molecular weight: 132 kDa.
For endogeneous detection (without G9a over expression): use always nuclear extracts and block overnight or perform IP prior to WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Intracellular
Developmental Biology >> Reproduction >> Germ cell markers
Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine methylation
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Stem Cells >> Embryonic Stem Cells >> Intracellular
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Relevance
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KMT1C / G9a is a histone methytransferase that preferentially methylates K9 and K27 of histone H3 in vitro. H3 K9 methylation serves as a mark for epigenetic transcriptional repression by recruiting HP1 to the site of this post-translational modification. The binding of HP1 also may serve to recruit Dnmt3a/b, resulting in DNA methylation. G9a-mediated histone methylation plays a significant role in epigenetic silencing.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KMT1C / G9a antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for KMT1C / G9a antibody - ChIP Grade images, references, products related to ab40542 and other tools.
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KMT1C / G9a antibody - ChIP Grade images:
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 Western blot - G9a antibody - ChIP Grade (ab40542)
All lanes : KMT1C / G9a antibody - ChIP Grade (ab40542) at 1/2500 dilution
Lane 1 : Untransfected HeLa cell extract
Lane 2 : G9a transfected HeLa cell extract
Predicted band size : 132 kDa
Observed band size : 132 kDa
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
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References for KMT1C / G9a antibody - ChIP Grade (ab40542)
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This product has been used in: (two most recent references)
Chen X et al. The NF-kappaB factor RelB and histone H3 lysine methyltransferase G9a directly interact to generate epigenetic silencing in endotoxin tolerance. J Biol Chem 284:27857-65 (2009). IP; Human. PubMed: 19690169
Stewart MD et al. Dynamic Regulation of Histone Modifications in Xenopus Oocytes through Histone Exchange. Mol Cell Biol 26:6890-901 (2006). PubMed: 16943430
If you publish research using ab40542 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to KMT1C / G9a
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KMT1C / G9a antibody - ChIP Grade - more information
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KMT1C / G9a antibody - ChIP Grade - other tools:
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