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Overview
Product name
Anti-KMT1C / G9a antibody
See all KMT1C / G9a products (3) ...
Description
Rabbit polyclonal to KMT1C / G9a
Tested applications
Cross reactivity
Reacts with
Mouse, Rat
Predicted to work with
Human
Immunogen
Synthetic peptide derived from within residues 1 - 100 of Mouse KMT1C / G9a.
(Peptide available as ab31873.)
Positive control
This antibody gave a positive result in the following lysates: Mouse EGL Whole Cell Lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Intracellular
Developmental Biology >> Reproduction >> Germ cell markers
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine methylation
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Stem Cells >> Embryonic Stem Cells >> Intracellular
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation
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Western blot - G9a antibody (ab31874)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 138 kDa (predicted molecular weight: 140 kDa).
Target
Relevance
KMT1C / G9a is a histone methytransferase that preferentially methylates K9 and K27 of histone H3 in vitro. H3 K9 methylation serves as a mark for epigenetic transcriptional repression by recruiting HP1 to the site of this post-translational modification. The binding of HP1 also may serve to recruit Dnmt3a/b, resulting in DNA methylation. G9a-mediated histone methylation plays a significant role in epigenetic silencing.
Cellular localization
Nuclear
Alternative names
- Bat 8 antibody
- Bat8 antibody
- EC 2.1.1.43 antibody
see all
Database links
- Entrez Gene: 10919 Human
- Entrez Gene: 110147 Mouse
- Entrez Gene: 361798 Rat
- Omim: 604599 Human
- SwissProt: Q96KQ7 Human
- SwissProt: Q9Z148 Mouse
- Unigene: 75196 Human
Anti-KMT1C / G9a antibody images:
Western blot - G9a antibody (ab31874)
Anti-KMT1C / G9a antibody (ab31874) at 1 µg/ml + Mouse EGL Whole Cell Lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 140 kDa
Observed band size : 140 kDa
Additional bands at : 170 kDa (possible isoform).
It is believed that the additional bands in the 170 kDa region are two splice variants (PMID: 12130538).
Tachibana M et al. G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis. Genes Dev 16:1779-91 (2002).
References for Anti-KMT1C / G9a antibody (ab31874)
This product has been referenced in:
- Sdek Pet al. Rb and p130 control cell cycle gene silencing to maintain the postmitotic phenotype in cardiac myocytes. J Cell Biol 194:407-23 (2011). WB; Mouse.Read more (PubMed: 21825075) »
- Davison JMet al. Gestational choline supply regulates methylation of histone H3, expression of histone methyltransferases G9a (Kmt1c) and Suv39h1 (Kmt1a), and DNA methylation of their genes in rat fetal liver and brain. J Biol Chem 284:1982-9 (2009). WB; Rat.Read more (PubMed: 19001366) »
See all 2 publications for this product
Publishing research using ab31874? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"