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Western blot - G9a antibody (ab31874)
(enlarge)
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Product Name
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KMT1C / G9a antibody
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See all KMT1C / G9a antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to KMT1C / G9a
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Immunogen
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Synthetic peptide derived from within residues 1 - 100 of Human G9a. (Peptide available as ab31873.)
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Reacts with
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Ms, Rat
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Predicted to react
(species key)
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Hu
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 138 kDa (predicted molecular weight: 140 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive result in the following lysates:
Mouse EGL Whole Cell Lysate
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Intracellular
Developmental Biology >> Reproduction >> Germ cell markers
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine methylation
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Stem Cells >> Embryonic Stem Cells >> Intracellular
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Relevance
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KMT1C / G9a is a histone methytransferase that preferentially methylates K9 and K27 of histone H3 in vitro. H3 K9 methylation serves as a mark for epigenetic transcriptional repression by recruiting HP1 to the site of this post-translational modification. The binding of HP1 also may serve to recruit Dnmt3a/b, resulting in DNA methylation. G9a-mediated histone methylation plays a significant role in epigenetic silencing.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.40 - 1.00 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KMT1C / G9a antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for KMT1C / G9a antibody images, references, products related to ab31874 and other tools.
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KMT1C / G9a antibody images:
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 Western blot - G9a antibody (ab31874)
KMT1C / G9a antibody (ab31874) at 1 µg/ml + Mouse EGL Whole Cell Lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 140 kDa
Observed band size : 140 kDa
Additional bands at : 170 kDa (possible isoform).
It is believed that the additional bands in the 170 kDa region are two splice variants (PMID: 12130538). Tachibana M et al. G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis. Genes Dev 16:1779-91 (2002).
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References for KMT1C / G9a antibody (ab31874)
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This product has been used in:
Davison JM et al. Gestational choline supply regulates methylation of histone H3, expression of histone methyltransferases G9a (Kmt1c) and Suv39h1 (Kmt1a), and DNA methylation of their genes in rat fetal liver and brain. J Biol Chem 284:1982-9 (2009). WB; Rat. PubMed: 19001366
If you publish research using ab31874 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to KMT1C / G9a
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KMT1C / G9a antibody - more information
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Customer reviews (feedback) regarding KMT1C / G9a antibody |
Customer FAQs regarding KMT1C / G9a antibody |
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KMT1C / G9a antibody - related products:
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KMT1C / G9a antibody - other tools:
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Contact Abcam with a Technical Enquiry about KMT1C / G9a antibody |
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