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Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225)

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Overview

Product name

Anti-KMT1E / SETDB1 antibody [5H6A12]
See all KMT1E / SETDB1 products (6) ...

Description

Mouse monoclonal [5H6A12] to KMT1E / SETDB1

Tested applications

WB, ICC/IF, ELISAmore details

Cross reactivity

Reacts with

Mouse, Human, Monkey

Immunogen

Purified recombinant fragment of human SETDB1 expressed in E. Coli.

Positive control

MCF-7,T47D, HEK293, JURKAT, NIH/3T3, F9, RAW246.7 and COS-7 cell lysates.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.03% Sodium Azide

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

5H6A12

Isotype

IgG1

Research areas

Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine methylation
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation

  • Western blot - KMT1E / SETDB1 antibody [5H6A12] (ab107225)Western blot - KMT1E / SETDB1 antibody [5H6A12] (ab107225) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - KMT1E / SETDB1 antibody [5H6A12] (ab107225)Immunocytochemistry/ Immunofluorescence - KMT1E / SETDB1 antibody [5H6A12] (ab107225) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab107225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/2000. Pred...Read more →

    WB: 1/500 - 1/2000. Predicted molecular weight: 180 kDa.

  • 1 Image

    ICC/IF

     ICC/IF: 1/200 - 1/1000.

    ICC/IF: 1/200 - 1/1000.

  • ELISA

     ELISA: 1/10000.

    ELISA: 1/10000.

Target

Function

Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.

Tissue specificity

Widely expressed. High expression in testis.

Sequence similarities

Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 1 MBD (methyl-CpG-binding) domain.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain.
Contains 2 Tudor domains.

Domain

The pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.
Isoform 2 lacks all domains required for histone methyltransferase activity.

Cellular localization

Nucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.

Target information above from: UniProt accessionQ15047 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • AU022152 antibody
  • EC 2.1.1.43 antibody
  • ERG-associated protein with SET domain antibody
  • ESET antibody
  • H3 K9 HMTase 4 antibody
  • H3-K9-HMTase 4 antibody
  • H3-K9-HMTase4 antibody
  • Histone H3 K9 methyltransferase 4 antibody
  • Histone H3-K9 methyltransferase 4 antibody
  • Histone-lysine N-methyltransferase SETDB1 antibody
  • KG1T antibody
  • KIAA0067 antibody
  • KMT1E antibody
  • Lysine N-methyltransferase 1E antibody
  • MGC90670 antibody
  • mKIAA0067 antibody
  • SET domain bifurcated 1 antibody
  • Set domain protein, bifurcated, 1 antibody
  • SETB1_HUMAN antibody
  • Setdb1 antibody
see all

Database links

Anti-KMT1E / SETDB1 antibody [5H6A12] images:

  Western blot - KMT1E / SETDB1 antibody [5H6A12] (ab107225)

Western blot - KMT1E / SETDB1 antibody [5H6A12] (ab107225)

All lanes : Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225) at 1/500 dilution

Lane 1 : MCF-7
Lane 2 : T47D
Lane 3 : HEK293
Lane 4 : JURKAT
Lane 5 : NIH/3T3
Lane 6 : F9
Lane 7 : RAW246.7
Lane 8 : COS-7


Predicted band size : 180 kDa

  Immunocytochemistry/ Immunofluorescence - KMT1E / SETDB1 antibody [5H6A12] (ab107225)

Immunocytochemistry/ Immunofluorescence - KMT1E / SETDB1 antibody [5H6A12] (ab107225)

Immunofluoresence detection of SETDB1 in LOVO cells using ab107225 (green). Actin fillaments have been labeled with Alexa Fluor-555 phalloidin (red).

References for Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225)

ab107225 has not yet been referenced specifically in any publications.

Publishing research using ab107225? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"