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Overview
Product name
Anti-KMT1E / SETDB1 antibody - ChIP Grade
See all KMT1E / SETDB1 products (6) ...
Description
Rabbit polyclonal to KMT1E / SETDB1 - ChIP Grade
Tested applications
ChIP, ICC/IF, WB, IHC-P, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Immunogen was a synthetic peptide, which represented a portion of human SET domain, bifurcated 1 encoded within exon 19 (LocusLink ID 9869).
Positive control
293T cells.
General notes
Centrifuge tube to remove product from lid.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified using the peptide immobilized on solid support.
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent concentration. (PubMed: 21921037)
WB: 1/1000 - 1/10000.Predicted molecular weight: 155 kDa.
IHC-P: Use at an assay dependent dilution.
IP: Use a concentration of 1 - 4 mg/ml.
Target
Function
Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.
Tissue specificity
Widely expressed. High expression in testis.
Sequence similarities
Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 1 MBD (methyl-CpG-binding) domain.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain.
Contains 2 Tudor domains.
Domain
The pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.
Isoform 2 lacks all domains required for histone methyltransferase activity.
Cellular localization
Nucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
Target information above from: UniProt accessionQ15047
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AU022152 antibody
- EC 2.1.1.43 antibody
- ERG-associated protein with SET domain antibody
see all
Database links
- Entrez Gene: 9869 Human
- Entrez Gene: 84505 Mouse
- Omim: 604396 Human
- SwissProt: Q15047 Human
- SwissProt: O88974 Mouse
- Unigene: 643565 Human
- Unigene: 479874 Mouse
Anti-KMT1E / SETDB1 antibody - ChIP Grade images:
Immunoprecipitation - SETDB1 antibody (ab12317)
Whole cell lysate (2 mg) from 293T cells that were mock transfected (1 and 2) or transfected with a SETDB1 expression construct (3 and 4). Rabbit anti-SETDB1 antibodies; ab12317 (2 and 4) and a competitor's antiserum (1 and 3) were used at 1 mcg/mg lysate for IP. ab12317 was used at 0.2 mcg/ml for Western Blot. Detection: Chemiluminescence with exposure times of 1 to 5 minutes.
Western blot - SETDB1 antibody (ab12317)
Predicted band size : 155 kDa
Samples: Whole cell lysate (10 mcg for 3 and 4; 50 mcg for 1 and 2) from 293T cells that were mock transfected (1 and 2) or transfected with a SETDB1 expression construct (3 and 4).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-KMT1E / SETDB1 antibody - ChIP Grade(ab12317)
ab12317 (4ug/ml) staining KMT1E in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of KMT1E in nuclear compartments of the germinal epithelium.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-KMT1E / SETDB1 antibody - ChIP Grade (ab12317)
This product has been referenced in:
- Mysliwiec MRet al. Jarid2 (Jumonji, AT Rich Interactive Domain 2) Regulates NOTCH1 Expression via Histone Modification in the Developing Heart. J Biol Chem 287:1235-41 (2012). ChIP; Mouse.Read more (PubMed: 22110129) »
- Cho Set al. Dual Functions of Histone-Lysine N-Methyltransferase Setdb1 Protein at Promyelocytic Leukemia-Nuclear Body (PML-NB): MAINTAINING PML-NB STRUCTURE AND REGULATING THE EXPRESSION OF ITS ASSOCIATED GENES. J Biol Chem 286:41115-24 (2011). WB, ICC/IF; Mouse.Read more (PubMed: 21921037) »
See all 8 publications for this product
Publishing research using ab12317? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"