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Overview
Product name
Anti-KMT1E / SETDB1 antibody - N-terminal
See all KMT1E / SETDB1 products (6) ...
Description
Rabbit polyclonal to KMT1E / SETDB1 - N-terminal
Tested applications
IHC-P, ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide (Human) conjugated to KLH
Positive control
Jurkat tissue lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab71194 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - KMT1E / SETDB1 antibody - N-terminal (ab71194)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
IHC-P: 1/10 - 1/50.IHC-P: 1/10 - 1/50.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/100 - 1/500.Predicted molecular weight: 143 kDa.
Target
Function
Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.
Tissue specificity
Widely expressed. High expression in testis.
Sequence similarities
Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 1 MBD (methyl-CpG-binding) domain.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain.
Contains 2 Tudor domains.
Domain
The pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.
Isoform 2 lacks all domains required for histone methyltransferase activity.
Cellular localization
Nucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
Target information above from: UniProt accessionQ15047
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AU022152 antibody
- EC 2.1.1.43 antibody
- ERG associated protein with a SET domain ESET antibody
see all
Database links
- Entrez Gene: 9869 Human
- Omim: 604396 Human
- SwissProt: Q15047 Human
- Unigene: 643565 Human
Anti-KMT1E / SETDB1 antibody - N-terminal images:
Western blot - KMT1E / SETDB1 antibody - N-terminal (ab71194)
All lanes : Anti-KMT1E / SETDB1 antibody - N-terminal (ab71194) at 1/500 dilution
Lane 1 : Cell lysates prepared from non trasfected 293 cells
Lane 2 : Cell lysates prepared from 293 cells transfected with SETDB1 gene
Predicted band size : 143 kDa
References for Anti-KMT1E / SETDB1 antibody - N-terminal (ab71194)
ab71194 has not yet been referenced specifically in any publications.
Publishing research using ab71194? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"