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Immunoprecipitation - SETDB1 antibody (ab12317)
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Western blot - SETDB1 antibody (ab12317)
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Product Name
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KMT1E / SETDB1 antibody - ChIP Grade
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See all KMT1E / SETDB1 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to KMT1E / SETDB1 - ChIP Grade
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Immunogen
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Immunogen was a synthetic peptide, which represented a portion of human SET domain, bifurcated 1 encoded within exon 19 (LocusLink ID 9869).
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ChIP, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use at an assay dependent dilution (PMID 15327775).
IP: Use at a concentration of 1 - 4 mg/ml.
WB: 1/1000 - 1/10000. Predicted molecular weight: 155 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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293T cells.
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Cellular localization
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Nuclear. Associated with nonpericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine methylation
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Relevance
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KMT1E / SETDB1 is a Histone methyltransferase which contains a C-terminal SET domain. This ~ 150-amino acid motif is implicated in the modulation of chromatin structure. SETBD1 specifically methylates Lys-9 of histone H3. This methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. SETDB1 is targeted to histone H3 by TRIM28/TIF1B and contributes to silencing of euchromatic genes by KRAB zinc-finger proteins. There are 2 human isoforms of KMT1E. Isoform 2 lacks all domains required for histone methyltransferase activity.
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KMT1E / SETDB1 antibody - ChIP Grade (ab12317) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: Tris-citrate/phosphate. pH 7 to 8. Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified using the peptide immobilized on solid support.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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Notes
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Centrifuge tube to remove product from lid.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KMT1E / SETDB1 antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for KMT1E / SETDB1 antibody - ChIP Grade images, references, products related to ab12317 and other tools.
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KMT1E / SETDB1 antibody - ChIP Grade images:
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 Immunoprecipitation - SETDB1 antibody (ab12317)
Whole cell lysate (2 mg) from 293T cells that were mock transfected (1 and 2) or transfected with a SETDB1 expression construct (3 and 4). Rabbit anti-SETDB1 antibodies; ab12317 (2 and 4) and a competitor's antiserum (1 and 3) were used at 1 mcg/mg lysate for IP. ab12317 was used at 0.2 mcg/ml for Western Blot. Detection: Chemiluminescence with exposure times of 1 to 5 minutes.
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Western blot - SETDB1 antibody (ab12317)
Predicted band size : 155 kDa
Samples: Whole cell lysate (10 mcg for 3 and 4; 50 mcg for 1 and 2) from 293T cells that were mock transfected (1 and 2) or transfected with a SETDB1 expression construct (3 and 4).
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References for KMT1E / SETDB1 antibody - ChIP Grade (ab12317)
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This product has been used in: (two most recent references)
Shirato H et al. A jumonji (Jarid2) protein complex represses cyclin D1 expression by methylation of histone H3-K9. J Biol Chem 284:733-9 (2009). WB; Human. PubMed: 19010785
Sarraf SA & Stancheva I Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly. Mol Cell 15:595-605 (2004). ChIP; Human. PubMed: 15327775
If you publish research using ab12317 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to KMT1E / SETDB1
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KMT1E / SETDB1 antibody - ChIP Grade - more information
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KMT1E / SETDB1 antibody - ChIP Grade - other tools:
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