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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot - KMT1E / SETDB1 antibody (ab5430)
Predicted band size : 143 kDa
Western blot using ab5430 at 1/1000 on 293 cell lysate.
30µg of lysate per lane.
Peptide competition (not shown) blocks staining of this band.
Predicted size is 143kD, but other antibodies also show staining at about 170kD.
Immunocytochemistry/ Immunofluorescence - KMT1E / SETDB1 antibody (ab5430)
ICC/IF image of ab5430 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5430, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
KMT1E / SETDB1 antibody for Immunoprecipitation in Human (5430)
KMT1E / SETDB1 antibody for ChIP in Mouse (5430)
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