Feedback Form

- KMT1E / SETDB1 antibody (ab5430)
- KMT1E / SETDB1 antibody (ab5430)
(enlarge)


Product Name 

KMT1E / SETDB1 antibody

 

See all KMT1E / SETDB1 antibodies (4)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to KMT1E / SETDB1

Immunogen 

Synthetic peptide: C-SRNYGYNPSPVKPEG conjugated to KLH, corresponding to amino acids 1058-1072 of Human ESET.
BLAST 'C-SRNYGYNPSPVKPEG' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Specificity 

Detects a band at 170kD in 293 cell lysate. This band can be blocked by the immunising peptide in WB. The predicted size is 143kD, however, several other antibodies detect bands at the same size as ab5430.

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews
(Read all Abreviews)

Submit an Abreview and earn up to 220 Abpoints by reviewing this product

• ChIP

1 Abreview

- average rating: 1 stars (out of 5)1 stars (out of 5)1 stars (out of 5)1 stars (out of 5)1 stars (out of 5)


ChIP
(read Abreview)


Application notes 
(see key)

Recommended dilutions

WB: 1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 143 kDa).

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear. Associated with nonpericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.

Research areas 

Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine methylation

Relevance 

KMT1E / SETDB1 is a Histone methyltransferase which contains a C-terminal SET domain. This ~ 150-amino acid motif is implicated in the modulation of chromatin structure. SETBD1 specifically methylates Lys-9 of histone H3. This methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. SETDB1 is targeted to histone H3 by TRIM28/TIF1B and contributes to silencing of euchromatic genes by KRAB zinc-finger proteins. There are 2 human isoforms of KMT1E. Isoform 2 lacks all domains required for histone methyltransferase activity.

 

KMT1E / SETDB1 antibody (ab5430)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

9869    (Human)

Omim

    

604396    (Human)

SwissProt

    

Q15047    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.170 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KMT1E / SETDB1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for KMT1E / SETDB1 antibody images, references, products related to ab5430 and other tools.

 

KMT1E / SETDB1 antibody images:

Product image  - KMT1E / SETDB1 antibody (ab5430)

- KMT1E / SETDB1 antibody (ab5430)



Predicted band size : 143 kDa


Western blot using ab5430 at 1/1000 on 293 cell lysate. 30µg of lysate per lane. Peptide competition (not shown) blocks staining of this band. Predicted size is 143kD, but other antibodies also show staining at about 170kD.

 
 

Search PubMed (MEDLINE) for references to KMT1E / SETDB1

 

KMT1E / SETDB1 antibody - more information

Customer reviews (feedback) regarding KMT1E / SETDB1 antibody

Customer FAQs regarding KMT1E / SETDB1 antibody

Protocols for KMT1E / SETDB1 antibody

Price and availability of products related to KMT1E / SETDB1 antibody

 

KMT1E / SETDB1 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Compatible Secondaries for KMT1E / SETDB1 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other KMT1E / SETDB1 Antibodies

See all KMT1E / SETDB1 antibodies (4)...

 

KMT1E / SETDB1 antibody - other tools:

Contact Abcam with a Technical Enquiry about KMT1E / SETDB1 antibody

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.