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- KMT1E / SETDB1 antibody (ab5430)
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Product Name
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KMT1E / SETDB1 antibody
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See all KMT1E / SETDB1 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to KMT1E / SETDB1
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Immunogen
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Synthetic peptide: C-SRNYGYNPSPVKPEG conjugated to KLH, corresponding to amino acids 1058-1072 of Human ESET.
BLAST 'C-SRNYGYNPSPVKPEG' with  or 
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Reacts with
(species key)
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Hu
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Specificity
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Detects a band at 170kD in 293 cell lysate. This band can be blocked by the immunising peptide in WB. The predicted size is 143kD, however, several other antibodies detect bands at the same size as ab5430.
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 143 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear. Associated with nonpericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine methylation
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Relevance
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KMT1E / SETDB1 is a Histone methyltransferase which contains a C-terminal SET domain. This ~ 150-amino acid motif is implicated in the modulation of chromatin structure. SETBD1 specifically methylates Lys-9 of histone H3. This methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. SETDB1 is targeted to histone H3 by TRIM28/TIF1B and contributes to silencing of euchromatic genes by KRAB zinc-finger proteins. There are 2 human isoforms of KMT1E. Isoform 2 lacks all domains required for histone methyltransferase activity.
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KMT1E / SETDB1 antibody (ab5430) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.170 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KMT1E / SETDB1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for KMT1E / SETDB1 antibody images, references, products related to ab5430 and other tools.
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KMT1E / SETDB1 antibody images:
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 - KMT1E / SETDB1 antibody (ab5430)
Predicted band size : 143 kDa
Western blot using ab5430 at 1/1000 on 293 cell lysate.
30µg of lysate per lane.
Peptide competition (not shown) blocks staining of this band.
Predicted size is 143kD, but other antibodies also show staining at about 170kD.
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Search PubMed (MEDLINE) for references to KMT1E / SETDB1
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KMT1E / SETDB1 antibody - more information
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Customer reviews (feedback) regarding KMT1E / SETDB1 antibody |
Customer FAQs regarding KMT1E / SETDB1 antibody |
Protocols for KMT1E / SETDB1 antibody |
Price and availability of products related to KMT1E / SETDB1 antibody |
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KMT1E / SETDB1 antibody - related products:
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KMT1E / SETDB1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about KMT1E / SETDB1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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