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Overview
Product name
Anti-KMT1E / SETDB1 antibody
See all KMT1E / SETDB1 products (6) ...
Description
Rabbit polyclonal to KMT1E / SETDB1
Specificity
Detects a band at 170kD in 293 cell lysate. This band can be blocked by the immunising peptide in WB. The predicted size is 143kD, however, several other antibodies detect bands at the same size as ab5430.
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: C-SRNYGYNPSPVKPEG conjugated to KLH, corresponding to amino acids 1058-1072 of Human ESET.
C-SRNYGYNP SPVKPEG
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
WB: 1/1000Detects a band of approximately 170 kDa (predicted molecular weight: 143 kDa).
Target
Function
Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.
Tissue specificity
Widely expressed. High expression in testis.
Sequence similarities
Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 1 MBD (methyl-CpG-binding) domain.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain.
Contains 2 Tudor domains.
Domain
The pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.
Isoform 2 lacks all domains required for histone methyltransferase activity.
Cellular localization
Nucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
Target information above from: UniProt accessionQ15047
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AU022152 antibody
- EC 2.1.1.43 antibody
- ERG-associated protein with SET domain antibody
see all
Database links
- Entrez Gene: 9869 Human
- Omim: 604396 Human
- SwissProt: Q15047 Human
- Unigene: 643565 Human
Anti-KMT1E / SETDB1 antibody images:
Western blot - KMT1E / SETDB1 antibody (ab5430)
Predicted band size : 143 kDa
Western blot using ab5430 at 1/1000 on 293 cell lysate.
30µg of lysate per lane.
Peptide competition (not shown) blocks staining of this band.
Predicted size is 143kD, but other antibodies also show staining at about 170kD.
Immunocytochemistry/ Immunofluorescence - KMT1E / SETDB1 antibody (ab5430)
ICC/IF image of ab5430 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5430, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-KMT1E / SETDB1 antibody (ab5430)
This product has been referenced in:
- Freiburghaus Cet al. Identification of ubiquitin in bovine milk and its growth inhibitory effects on human cancer cell lines. J Dairy Sci 93:3442-52 (2010).Read more (PubMed: 20655412) »
See 1 publication for this product
Publishing research using ab5430? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"