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Overview
Product name
Anti-KMT1E / SETDB1 antibody
See all KMT1E / SETDB1 products (6) ...
Description
Mouse monoclonal to KMT1E / SETDB1
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment with tag: SSLPGCIGLD AATATVESEE IAELQQAVVE ELGISMEELR HFIDEELEKM DCVQQRKKQL AELETWVIQK ESEVAHVDQL FDDASRAVTN CESLVKDFY, corresponding to amino acids 2-101 of Human SETDB1
SSLPGCIGLD AATATVESEE IAELQQAVVE ELGISMEELR HFIDEELEKM DCVQQRKKQL AELETWVIQK ESEVAHVDQL FDDASRAVTN CESLVKDFY
Positive control
SW13 whole cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2a
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
WB: Use a concentration of 1 - 5 µg/ml.Detects a band of approximately 143 kDa (predicted molecular weight: 143 kDa).
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Target
Function
Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.
Tissue specificity
Widely expressed. High expression in testis.
Sequence similarities
Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 1 MBD (methyl-CpG-binding) domain.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain.
Contains 2 Tudor domains.
Domain
The pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.
Isoform 2 lacks all domains required for histone methyltransferase activity.
Cellular localization
Nucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
Target information above from: UniProt accessionQ15047
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AU022152 antibody
- EC 2.1.1.43 antibody
- ERG associated protein with a SET domain ESET antibody
see all
Database links
- Entrez Gene: 9869 Human
- Omim: 604396 Human
- SwissProt: Q15047 Human
- Unigene: 643565 Human
Anti-KMT1E / SETDB1 antibody images:
Western blot - SETDB1 antibody (ab61329)
Anti-KMT1E / SETDB1 antibody (ab61329) at 1 µg/ml + SW13 whole cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 143 kDa
Observed band size : 143 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-KMT1E / SETDB1 antibody(ab61329)
IHC image of ab61329 staining in normal human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab61329, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-KMT1E / SETDB1 antibody (ab61329)
ab61329 has not yet been referenced specifically in any publications.
Publishing research using ab61329? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"