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Immunohistochemistry (Frozen sections) - ASH1 antibody (ab4477)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ASH1 antibody (ab4477)
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Product Name
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KMT2H / ASH1 antibody - ChIP Grade
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See all KMT2H / ASH1 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to KMT2H / ASH1 - ChIP Grade
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Immunogen
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Synthetic peptide derived from residues 1650 - 1750 of Human ASH1 conjugated to KLH.
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Reacts with
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Hu, Ms, Corn
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Predicted to react
(species key)
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Dm
(Due to sequence homology)
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Tested applications
(see key)
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ChIP, IHC-Fr, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use at an assay dependent dilution (PMID 17641388).
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol (See image legend for further details).
IHC-Fr: 1/100.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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embryonic day 12.5 mouse brain
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Cellular localization
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Nucleus. Cell junction; tight junction. The relevance of tight junction localization is however unclear.
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Chromatin Remodeling >> Trithorax
Neuroscience >> Neurology process >> Neurogenesis
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Relevance
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KMT2H / ASH1 (absent, small, or homeotic 1) and Trithorax (trx) are members of the Drosophila trx group. Their products are associated with chromosomes and are believed to activate transcription of target genes through chromatin remodeling.
KMT2H / ASH1 is expressed in multiple tissues. The protein contains a SET domain, a PHD finger, four AT hooks and a region with homology to the bromodomain.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KMT2H / ASH1 antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for KMT2H / ASH1 antibody - ChIP Grade images, references, products related to ab4477 and other tools.
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KMT2H / ASH1 antibody - ChIP Grade images:
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 Immunohistochemistry (Frozen sections) - ASH1 antibody (ab4477)
Immunofluorescent staining for ASH1 on mouse neocortex (E12.5 whole brain frozen sections) using Rabbit polyclonal to ASH1 (ab4477). Frozen brain sections were post-fixed in paraformaldehyde; blocking was performed with 5% milk (30mins). ab4477 was used at a dilution of 1/100, incubated for 14h at 4°C. Secondary antibody was Alexa fluor 555 (1/500). Speckled expression of ASH1 was observed in the nuclei of cells throughout the developing CNS, neocortex pictured as reported in the literature [PMID: 10860993] Dean Griffiths, Org unknown, Country unknown
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ASH1 antibody (ab4477)
IHC image of ab4477 stained human hippocampus FFPE section. Section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30 seconds at 125°C. Section was incubated with ab4477, 1µg/ml, for 1h at room temperature and detected using an HRP conjugated polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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References for KMT2H / ASH1 antibody - ChIP Grade (ab4477)
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This product has been used in: (two most recent references)
Casati P et al. Histone acetylation and chromatin remodeling are required for UV-B-dependent transcriptional activation of regulated genes in maize. Plant Cell 20:827-42 (2008). ChIP; Corn. PubMed: 18398050
Gregory GD et al. Mammalian ASH1L is a histone methyltransferase that occupies the transcribed region of active genes. Mol Cell Biol 27:8466-79 (2007). PubMed: 17923682
See all 3 publication references for this product.
If you publish research using ab4477 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to KMT2H / ASH1
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KMT2H / ASH1 antibody - ChIP Grade - more information
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