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Overview
Product name
Anti-SETD7 antibody - C-terminal
See all SETD7 products (9) ...
Description
Rabbit polyclonal to SETD7 - C-terminal
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide from the C terminal region of human KMT7 / SETD7/ SET7/9 conjugated to KLH.
Positive control
Mouse brain tissue lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab71214 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - KMT7 / SETD7/ SET7/9 antibody - C-terminal (ab71214)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/100 - 1/500.Detects a band of approximately 37 kDa (predicted molecular weight: 41 kDa).
Target
Function
Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro.
Tissue specificity
Widely expressed. Expressed in pancreatic islets.
Sequence similarities
Belongs to the histone-lysine methyltransferase family. SET7 subfamily.
Contains 3 MORN repeats.
Contains 1 SET domain.
Domain
The SET domain is necessary but not sufficient for histone methyltransferase activity.
Cellular localization
Nucleus. Chromosome.
Target information above from: UniProt accessionQ8WTS6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ21193 antibody
- H3 K4 HMTase antibody
- H3-K4-HMTase SETD7 antibody
see all
Database links
- Entrez Gene: 80854 Human
- Entrez Gene: 73251 Mouse
- Omim: 606594 Human
- SwissProt: Q8WTS6 Human
- SwissProt: Q8VHL1 Mouse
- Unigene: 480792 Human
- Unigene: 192111 Mouse
Anti-SETD7 antibody - C-terminal images:
Western blot - KMT7 / SETD7/ SET7/9 antibody - C-terminal (ab71214)
Anti-SETD7 antibody - C-terminal (ab71214) at 1/70 dilution + Mouse brain tissue lysate at 35 µg
Predicted band size : 41 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
References for Anti-SETD7 antibody - C-terminal (ab71214)
ab71214 has not yet been referenced specifically in any publications.
Publishing research using ab71214? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"