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Overview
Product name
Anti-SETD7 antibody - ChIP Grade
See all SETD7 products (9) ...
Description
Rabbit polyclonal to SETD7 - ChIP Grade
Tested applications
ChIP, WBmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse
Immunogen
Synthetic peptide (Human).
Positive control
Hela cell lysate.
General notes
ab13731 does not appear to work in IF using mouse or human cell lines.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 0.2% Gelatin, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
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Western blot - Anti-SETD7 antibody - ChIP Grade (ab13731)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP
Application notes
ChIP: Use at an assay dependent dilution (PMID 17578910).
WB: 1/500 - 1/1000. Detects a band of approximately 43 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro.
Tissue specificity
Widely expressed. Expressed in pancreatic islets.
Sequence similarities
Belongs to the histone-lysine methyltransferase family. SET7 subfamily.
Contains 3 MORN repeats.
Contains 1 SET domain.
Domain
The SET domain is necessary but not sufficient for histone methyltransferase activity.
Cellular localization
Nucleus. Chromosome.
Target information above from: UniProt accessionQ8WTS6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ21193 antibody
- H3 K4 HMTase antibody
- H3-K4-HMTase SETD7 antibody
see all
Database links
- Entrez Gene: 80854 Human
- Omim: 606594 Human
- SwissProt: Q8WTS6 Human
- Unigene: 480792 Human
Anti-SETD7 antibody - ChIP Grade images:
References for Anti-SETD7 antibody - ChIP Grade (ab13731)
This product has been referenced in:
- Narayanan Aet al. The coactivator host cell factor-1 mediates Set1 and MLL1 H3K4 trimethylation at herpesvirus immediate early promoters for initiation of infection. Proc Natl Acad Sci U S A 104:10835-40 (2007). ChIP; Human.Read more (PubMed: 17578910) »
See 1 publication for this product
Publishing research using ab13731? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"