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Anti-SETD7 antibody (ab3826)

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Overview

Product name

Anti-SETD7 antibody
See all SETD7 products (9) ...

Description

Rabbit polyclonal to SETD7

Specificity

The antibody recognises the Set9 protein in yeast extract and also recognises the recombinant his tagged protein. The band is not detected in the set9 deletion mutant.

Tested applications

WBmore details

Cross reactivity

Reacts with

Schizosaccharomyces pombe

Immunogen

Recombinant full length protein of S. pombe SET7 /SET9.

General notes

Accession NP_588078 (SPCC4B3.12)

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

IgG fraction

Primary antibody notes

This antibody was used to demonstrate that a previously uncharacterized SET domain protein, Set9, is responsible for H4-K20 methylation in the fission yeast Schizosaccharomyces pombe. Surprisingly, H4-K20 methylation does not have any apparent role in the regulation of gene expression or heterochromatin function. Rather, we find the modification has a role in DNA damage response. Loss of Set9 activity or mutation of H4-K20 markedly impairs cell survival after genotoxic challenge and compromises the ability of cells to maintain checkpoint mediated cell cycle arrest. Genetic experiments link Set9 to Crb2, a homolog of the mammalian checkpoint protein 53BP1, and the enzyme is required for Crb2 localization to sites of DNA damage. These results argue that H4-K20 methylation functions as a "histone mark" required for the recruitment of the checkpoint protein Crb2.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - KMT7 / SETD7/ SET7/9 antibody (ab3826)Western blot - KMT7 / SETD7/ SET7/9 antibody (ab3826) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab3826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

Target

Function

Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro.

Tissue specificity

Widely expressed. Expressed in pancreatic islets.

Sequence similarities

Belongs to the histone-lysine methyltransferase family. SET7 subfamily.
Contains 3 MORN repeats.
Contains 1 SET domain.

Domain

The SET domain is necessary but not sufficient for histone methyltransferase activity.

Cellular localization

Nucleus. Chromosome.

Target information above from: UniProt accessionQ8WTS6 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • FLJ21193 antibody
  • H3 K4 HMTase antibody
  • H3-K4-HMTase SETD7 antibody
  • H4 lysine 4 specific antibody
  • H4 lysine-4 specific antibody
  • Histone H3 K4 methyltransferase antibody
  • Histone H3 lysine 4 specific methyltransferase antibody
  • Histone H3-K4 methyltransferase SETD7 antibody
  • Histone H4 K4 methyltransferase antibody
  • Histone H4-K4 methyltransferase antibody
  • Histone lysine N methyltransferase antibody
  • Histone lysine N methyltransferase H3 lysine 4 specific SET7 antibody
  • Histone-lysine N-methyltransferase antibody
  • Histone-lysine N-methyltransferase SETD7 antibody
  • KIAA1717 antibody
  • KMT7 antibody
  • Lysine methyltransferase antibody
  • Lysine N-methyltransferase 7 antibody
  • OTTHUMP00000164543 antibody
  • OTTHUMP00000220049 antibody
  • SET 7 antibody
  • SET 7/9 antibody
  • SET 9 antibody
  • SET D7 antibody
  • SET domain containing (lysine methyltransferase) 7 antibody
  • SET domain containing protein 7 antibody
  • SET domain containing protein 8 antibody
  • SET domain-containing protein 7 antibody
  • SET domain-containing protein 8 antibody
  • SET domain-containing protein 8 antibody
  • SET7 antibody
  • SET7/9 antibody
  • SET9 antibody
  • SETD7 antibody
  • SETD7_HUMAN antibody
see all

Anti-SETD7 antibody images:

  Western blot - KMT7 / SETD7/ SET7/9 antibody (ab3826)

Western blot - KMT7 / SETD7/ SET7/9 antibody (ab3826)



Predicted band size : 55 kDa


Lysate: Fission yeast extract

Lane 1: Wildtype
Lane 2: Set9 deletion
Lane 3: His - 6 - tagged Set9 (1-5 ng).
Dilution of the antibody: 1 /1000.

Size of the band is 55 kDa.

Steven Sanders, University of Cambridge, UK

References for Anti-SETD7 antibody (ab3826)

This product has been referenced in:

  • Wang Yet al. Regulation of Set9-mediated H4K20 methylation by a PWWP domain protein. Mol Cell 33:428-37 (2009). WB; Schizosaccharomyces pombe.Read more (PubMed: 19250904) »
  • Sanders SLet al. Methylation of histone H4 lysine 20 controls recruitment of Crb2 to sites of DNA damage. Cell 119:603-14 (2004).Read more (PubMed: 15550243) »

See all 2 publications for this product

Publishing research using ab3826? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"