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Western blot - KMT7 / SETD7/ SET7/9 antibody (ab3826)
Western blot - KMT7 / SETD7/ SET7/9 antibody (ab3826)
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Product Name 

KMT7 / SETD7/ SET7/9 antibody

 

See all KMT7 / SETD7/ SET7/9 antibodies (6)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to KMT7 / SETD7/ SET7/9

Immunogen 

Recombinant full length protein of S. pombe SET7 /SET9.

Reacts with 
(species key)

S pom

Specificity 

The antibody recognises the Set9 protein in yeast extract and also recognises the recombinant his tagged protein. The band is not detected in the set9 deletion mutant.

Tested applications 
(see key)

WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
WB: 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Methylation >> Lysine methylation

Relevance 

KMT7 / SETD7 / SET7/9 is a histone methyltransferase responsible for the methylation of histone H3 K4, p53 and TAF10.

This antibody was used to demonstrate that a previously uncharacterized SET domain protein, Set9, is responsible for H4-K20 methylation in the fission yeast Schizosaccharomyces pombe.

Surprisingly, H4-K20 methylation does not have any apparent role in the regulation of gene expression or heterochromatin function. Rather, we find the modification has a role in DNA damage response. Loss of Set9 activity or mutation of H4-K20 markedly impairs cell survival after genotoxic challenge and compromises the ability of cells to maintain checkpoint mediated cell cycle arrest. Genetic experiments link Set9 to Crb2, a homolog of the mammalian checkpoint protein 53BP1, and the enzyme is required for Crb2 localization to sites of DNA damage. These results argue that H4-K20 methylation functions as a "histone mark" required for the recruitment of the checkpoint protein Crb2.

 

Alternative names KMT7 / SETD7/ SET7/9 antibody (ab3826)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Accession NP_588078 (SPCC4B3.12)

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this KMT7 / SETD7/ SET7/9 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for KMT7 / SETD7/ SET7/9 antibody images, references, products related to ab3826 and other tools.

 

KMT7 / SETD7/ SET7/9 antibody images:

Product image  Western blot - KMT7 / SETD7/ SET7/9 antibody (ab3826)

WB using ab3826

Western blot - KMT7 / SETD7/ SET7/9 antibody (ab3826)



Predicted band size : 55 kDa


Lysate: Fission yeast extract

Lane 1: Wildtype
Lane 2: Set9 deletion
Lane 3: His - 6 - tagged Set9 (1-5 ng).
Dilution of the antibody: 1 /1000.

Size of the band is 55 kDa.

Steven Sanders, University of Cambridge, UK

 

References for KMT7 / SETD7/ SET7/9 antibody (ab3826)

Specific publication references   This product has been used in: (two most recent references)

Wang Y et al. Regulation of Set9-mediated H4K20 methylation by a PWWP domain protein. Mol Cell 33:428-37 (2009). WB; Schizosaccharomyces pombe. PubMed: 19250904

Sanders SL et al. Methylation of histone H4 lysine 20 controls recruitment of Crb2 to sites of DNA damage. Cell 119:603-14 (2004). PubMed: 15550243
If you publish research using ab3826 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to KMT7 / SETD7/ SET7/9

 

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