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Products:Microbiology >> Organism >> Virus >> DNA Virus >> double stranded DNA Virus >> Other dsDNA viruses
Anti-KSHV ORF45 antibody [2D4A5] (ab36618)
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab36618? Please let us know so that we can cite the reference in this datasheet
ab36618 has been referenced in 1 publications.
- Abada R et al. SIAH-1 interacts with the Kaposi's sarcoma-associated herpesvirus-encoded ORF45 protein and promotes its ubiquitylation and proteasomal degradation. J Virol 82:2230-40 (2008). WB. PubMed: 18077711
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - KSHV ORF45 antibody [2D4A5] (ab36618)](/ps/datasheet/Images/36/ab36618/ab36618_WB.gif)
Western blot - KSHV ORF45 antibody [2D4A5] (ab36618)
All lanes : Anti-KSHV ORF45 antibody [2D4A5] (ab36618)
Lane 1 : Molecular weight marker
Lane 2 : Recombinant KSHV ORF45 expressed in E.coli
Predicted band size : 50 kDa
Observed band size : 78 kDa (why is the actual band size different from the predicted?)
Based on the analysis of the mRNA sequence, ORF45 is expected to encode a protein of 407 amino acids with a molecular mass of about 50 kDa. However, ORF45 was displayed as a diffuse band at the position of 78 kDa on a Western blot after SDS-PAGE. This observation implied that ORF45 might be highly post-translationally modified, resulting in its reduced mobility in denaturing SDS-PAGE. A survey of the primary amino acid sequence of ORF45 revealed multiple potential sites of phosphorylation, including a serine-rich region and other consensus sites for phosphorylation by the serine/threonine kinase, casein kinase II, and protein kinase C (Zhu & Yuan, 2003).
![Immunocytochemistry - KSHV ORF45 antibody [2D4A5] (ab36618)](/ps/datasheet/Images/36/ab36618/ab36618_ICC.gif)
Immunocytochemistry - KSHV ORF45 antibody [2D4A5] (ab36618)
Immunochemistry staining of TPA-induced BCBL-1 cells, using ab36618. HRP-anti-mouse was used as the secondary antibody before colour development with DAB.
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