![Western blot - KSHV ORF45 antibody [2D4A5] (ab36618) image](#ab36618_WB.gif)
![Immunocytochemistry - KSHV ORF45 antibody [2D4A5] (ab36618) image](#ab36618_ICC.gif)
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Products:Microbiology >> Organism >> Virus >> DNA Virus >> double stranded DNA Virus >> Other dsDNA viruses
Overview
Product name
Anti-KSHV ORF45 antibody [2D4A5]
Description
Mouse monoclonal [2D4A5] to KSHV ORF45
Tested applications
WB, ELISAmore details
Cross reactivity
Kaposi's sarcoma herpes virus
Immunogen
Ni-NTA purified recombinant human KSHV ORF45 expressed in E. Coli strain BL21 (DE3).
Positive control
TPA induced BCBL-1 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Constituents: Ascites
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
2D4A5
Isotype
IgG1
Research areas
Microbiology >> Organism >> Virus >> DNA Virus >> double stranded DNA Virus >> Other dsDNA viruses
Applications
Show applications key
Our Abpromise guarantee covers the use of ab36618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 78 kDa (predicted molecular weight: 50 kDa).
ELISA: Use at an assay dependent dilution.
Target
Relevance
Kaposi's sarcoma-associated herpes virus (KSHV) belongs to the gamma-herpesvirus subfamily of the Herpes viridae family and has been closely linked to the Kaposi's sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman's disease. The genome of KSHV is 165-170 kb and contains at least 88 open reading frames. ORF45 protein encoded by an immediate-early gene in the KSHV genome is characterized as a phosphorylated protein, and it is localized in the cytoplasm of infected cells. Studies have shown that ORF45 Protein interacted with cellular IRF-7 and blocked virus-mediated phosphorylation and nuclear translocation of IRF-7. In consequence, ORF45 efficiently inhibited virus-induced production of type I IFN. Zhu et al (2003) reported that the ORF45 Protein was associated with purified virions.
Cellular localization
Not applicable
Alternative names
- HHV8 antibody
- Human herpes virus 8 antibody
- Kaposi's sarcoma herpes virus antibody
see all
Anti-KSHV ORF45 antibody [2D4A5] images:
Western blot - KSHV ORF45 antibody [2D4A5] (ab36618)
![Western blot - KSHV ORF45 antibody [2D4A5] (ab36618)](/ps/datasheet/Images/36/ab36618/ab36618_WB.gif)
All lanes : Anti-KSHV ORF45 antibody [2D4A5] (ab36618)
Lane 1 : Molecular weight marker
Lane 2 : Recombinant KSHV ORF45 expressed in E.coli
Predicted band size : 50 kDa
Observed band size : 78 kDa (why is the actual band size different from the predicted?)
Based on the analysis of the mRNA sequence, ORF45 is expected to encode a protein of 407 amino acids with a molecular mass of about 50 kDa. However, ORF45 was displayed as a diffuse band at the position of 78 kDa on a Western blot after SDS-PAGE. This observation implied that ORF45 might be highly post-translationally modified, resulting in its reduced mobility in denaturing SDS-PAGE. A survey of the primary amino acid sequence of ORF45 revealed multiple potential sites of phosphorylation, including a serine-rich region and other consensus sites for phosphorylation by the serine/threonine kinase, casein kinase II, and protein kinase C (Zhu & Yuan, 2003).
Immunocytochemistry - KSHV ORF45 antibody [2D4A5] (ab36618)
![Immunocytochemistry - KSHV ORF45 antibody [2D4A5] (ab36618)](/ps/datasheet/Images/36/ab36618/ab36618_ICC.gif)
Immunochemistry staining of TPA-induced BCBL-1 cells, using ab36618. HRP-anti-mouse was used as the secondary antibody before colour development with DAB.
References for Anti-KSHV ORF45 antibody [2D4A5] (ab36618)
This product has been referenced in:
- Abada Ret al. SIAH-1 interacts with the Kaposi's sarcoma-associated herpesvirus-encoded ORF45 protein and promotes its ubiquitylation and proteasomal degradation. J Virol 82:2230-40 (2008). WB.Read more (PubMed: 18077711) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"