Kaiso antibody [2T15] (ab11810)

Product Name 

Kaiso antibody [2T15]

Product type 

Primary antibodies

Description 

Mouse monoclonal [2T15] to Kaiso

Immunogen 

Recombinant fusion protein (N-terminal), corresponding to amino acids 103 - 584 of mouse Kaiso.

Reacts with 
(species key)

Ms, Rat, Dog, Hm

Tested applications 
(see key)

GSA, ICC, IP, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
GSA: Use at an assay dependent dilution. Supershifts a specific methyl-CpG complex.
ICC: Use at a concentration of 1 µg/ml for positive nuclear staining in NIH3T3 cells fixed with 100% methanol.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 0.5 - 2 µg/ml on RIPA lysates from 3T3/A31 cells (ECL). Detects a band of approximately 97 kDa (predicted molecular weight: 75 kDa).

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

3T3/A31 cell lysate. Add 2.5µl of 2-mercaptoethanol/100µl of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Co-factors
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> Methylated DNA

Relevance 

Kaiso is Caribbean slang for calypso music, which accompanied the late-night cloning of this gene. The deduced 672-amino acid human protein is 87% identical to the mouse protein, with mostly conservative changes. Kaiso contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. Immunoprecipitation analysis showed that Kaiso interacts with Ctnnd1 but not with Ctnnb1, Ctnna1 or Cdh1. Immunofluorescence analysis demonstrated a diffuse nuclear localization. Northern blot analysis of mouse tissues revealed ubiquitous expression of 5 to 6kb transcripts. Yeast 2 hybrid analysis indicated that the armadillo repeats of Ctnnd1 interact with a region flanking the zinc finger domain of Kaiso. By genomic sequence analysis, Daniel and Reynolds (1999) mapped the human KAISO gene to Xq23. They mapped the mouse Kaiso gene to the X chromosome also.

Kaiso is a component of the human N-CoR complex. In vitro, the Kaiso/N-CoR complex binds specific CpG-rich sequences in a methylation-dependent manner. In vivo, Kaiso targets the N-CoR complex to the MTA2 gene promoter in a methylation-dependent manner. Kaiso is required for transcriptional repression of the methylated MTA2 locus. Furthermore, this repression also requires a functional N-CoR deacetylase complex, which brings about histone hypoacetylation and methylation of H3 lysine 9 to the MTA2 locus (Yoon HG et al. 2003).

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

2T15

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 30% Glycerol, 0.15M Sodium Chloride, 0.1M Tris-glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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