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Overview
Product name
Anti-Kaiso antibody [2T15]
See all Kaiso products (3) ...
Description
Mouse monoclonal [2T15] to Kaiso
Tested applications
ICC, WB, Gel supershift assays, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Hamster, Dog
Predicted to work with
Chicken, Human
Immunogen
Recombinant fusion protein (N-terminal), corresponding to amino acids 103 - 584 of mouse Kaiso.
Positive control
3T3/A31 cell lysate. Add 2.5µl of 2-mercaptoethanol/100µl of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
General notes
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 30% Glycerol, 0.15M Sodium Chloride, 0.1M Tris-glycine. pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
2T15
Isotype
IgG1
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC
WB
Gel supershift assays
IP
Application notes
GSA: Use at an assay dependent dilution. Supershifts a specific methyl-CpG complex.
ICC: Use at a concentration of 1 µg/ml for positive nuclear staining in NIH3T3 cells fixed with 100% methanol.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 0.5 - 2 µg/ml on RIPA lysates from 3T3/A31 cells (ECL). Detects a band of approximately 97 kDa (predicted molecular weight: 75 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2.
Tissue specificity
Expressed in vascular endothelium.
Sequence similarities
Contains 1 BTB (POZ) domain.
Contains 3 C2H2-type zinc fingers.
Cellular localization
Nucleus. Cytoplasm. Also cytoplasmic in cells grown at high densities.
Target information above from: UniProt accessionQ86T24
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Zinc finger and BTB domain containing 33 antibody
- Kaiso antibody
- Kaiso protein antibody
see all
Database links
- Entrez Gene: 10009 Human
- Entrez Gene: 56805 Mouse
- Omim: 300329 Human
- SwissProt: Q86T24 Human
- SwissProt: Q8BN78 Mouse
- SwissProt: Q9WTZ7 Mouse
- Unigene: 143604 Human
- Unigene: 391248 Mouse
References for Anti-Kaiso antibody [2T15] (ab11810)
ab11810 has not yet been referenced specifically in any publications.
Publishing research using ab11810? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"