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Anti-Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)

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1 question for ab12723

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Question 1

Tuesday 13-December-2011

The immunogen says, "His-tagged Kaiso fusion protein (Human) lacking the zinc finger region."  Do you have the actual residues?  According to Swiss Prot, the region of a.a. 514 - 638 is required for DNA binding.  Does this work in ChIP because this region is included in the immunogen?  

ANSWER:

  

Thank you for your inquiry.

I heard back from our lab that unfortunately we do not have any more information on the immunogen sequence other than what is stated on the datasheet.   

Ideally,  for an antibody that works in ChIP, the immunogen sequence should avoid the DNA binding domain.  This is because if the target protein has bound to the DNA, this region is now blocked and no longer accessible to the antibody.  For this reason we also try to avoid designing immunogens to the activation domain too.

 I hope this information helps.  Please contact us with any other questions.  

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