Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)

Product Name 

Kaiso antibody [6F / 6F8] - ChIP Grade

Product type 

Primary antibodies

Description 

Mouse monoclonal [6F / 6F8] to Kaiso - ChIP Grade

Immunogen 

His-tagged Kaiso fusion protein (Human) lacking the zinc finger region.

Reacts with 
(species key)

Hu, Ms, Rat, Chk, Dog, Hm

Tested applications 
(see key)

ChIP, ICC/IF, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ChIP: Use at an assay dependent dilution.
IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 80 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

CSML0 cells (IP then Western)

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Transcription >> Co-factors
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> Methylated DNA

Relevance 

Kaiso is Caribbean slang for calypso music, which accompanied the late-night cloning of this gene. The deduced 672-amino acid human protein is 87% identical to the mouse protein, with mostly conservative changes. Kaiso contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. Immunoprecipitation analysis showed that Kaiso interacts with Ctnnd1 but not with Ctnnb1, Ctnna1 or Cdh1. Immunofluorescence analysis demonstrated a diffuse nuclear localization. Northern blot analysis of mouse tissues revealed ubiquitous expression of 5 to 6kb transcripts. Yeast 2 hybrid analysis indicated that the armadillo repeats of Ctnnd1 interact with a region flanking the zinc finger domain of Kaiso. By genomic sequence analysis, Daniel and Reynolds (1999) mapped the human KAISO gene to Xq23. They mapped the mouse Kaiso gene to the X chromosome also.

Kaiso is a component of the human N-CoR complex. In vitro, the Kaiso/N-CoR complex binds specific CpG-rich sequences in a methylation-dependent manner. In vivo, Kaiso targets the N-CoR complex to the MTA2 gene promoter in a methylation-dependent manner. Kaiso is required for transcriptional repression of the methylated MTA2 locus. Furthermore, this repression also requires a functional N-CoR deacetylase complex, which brings about histone hypoacetylation and methylation of H3 lysine 9 to the MTA2 locus (Yoon HG et al. 2003).

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

6F / 6F8

Isotype 

IgG1

Purity 

IgG fraction

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Kaiso antibody [6F / 6F8] - ChIP Grade images, references, products related to ab12723 and other tools.

Kaiso antibody [6F / 6F8] - ChIP Grade images:

References for Kaiso antibody [6F / 6F8] - ChIP Grade (ab12723)

  This product has been used in: (two most recent references)

Rodova M et al. Regulation of the rapsyn promoter by kaiso and delta-catenin. Mol Cell Biol 24:7188-96 (2004). PubMed: 15282317

Daniel JM et al. Monoclonal antibodies to Kaiso: a novel transcription factor and p120ctn-binding protein. Hybridoma 20:159-66 (2001). PubMed: 11461664

See all 3 publication references for this product.


If you publish research using ab12723 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding Kaiso antibody [6F / 6F8] - ChIP Grade

Customer FAQs regarding Kaiso antibody [6F / 6F8] - ChIP Grade

Protocols for Kaiso antibody [6F / 6F8] - ChIP Grade

Price and availability of products related to Kaiso antibody [6F / 6F8] - ChIP Grade

Kaiso antibody [6F / 6F8] - ChIP Grade - related products: