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Anti-Kallikrein 1 antibody - Catalytic domain (ab40947)

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Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab40947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 29 kDa. Dilution optimised using Chromogenic detection. A range of different sized bands can be seen for Kallikrein 1 in different tissues and in cell culture media, ranging from 16-38 kDa and likely representing different proteolytically processed forms. SDS-PAGE: Use at an assay dependent dilution. Extensive glycosylation and other posttranslational modifications give Kallikrein an apparent mass of 36-46 kDa on SDS PAGE gels. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionGlandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
    • Tissue specificityIsoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84.
    • Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
      Contains 1 peptidase S1 domain.
    • Post-translational
      modifications      The O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located.

      • Target information above from: UniProt accession P06870 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          Glandular kallikrein 1 antibodyhK 1 antibodyhK1 antibody
          hK1 antibodyKallikrein serine protease 1 antibodyKallikrein-1 antibodyKidney/pancreas/salivary gland kallikrein antibodyKidney/pancreas/salivary gland kallikrein antibodyKlk 6 antibodyKLK1 antibodyKLK1_HUMAN antibodyKlk6 antibodyKLKR antibodyTissue kallikrein antibody
        see all

      Anti-Kallikrein 1 antibody - Catalytic domain images

      • Western blot - Kallikrein 1 antibody - Catalytic domain (ab40947)
        Western blot - Kallikrein 1 antibody - Catalytic domain (ab40947)
        All lanes : Anti-Kallikrein 1 antibody - Catalytic domain (ab40947) at 1/1000 dilution

        Lane 1 : Kallikrein 1 at 0.05 µg
        Lane 2 : Kallikrein 1 at 0.01 µg
        Lane 3 : Kallikrein 1 at 0.001 µg


        Predicted band size : 29 kDa
        Observed band size : 38,46 kDa (why is the actual band size different from the predicted?)

      References for Anti-Kallikrein 1 antibody - Catalytic domain (ab40947)

      ab40947 has not yet been referenced specifically in any publications.

      Publishing research using ab40947 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab40947 Submit a Question for ab40947

      Displaying 1 - 2 of 2 results for Abreviews and Q&A

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      Are these antibodies likely to cross-react with mouse proteins?

      Following up my previous message, immunogens for the kallikrein antibodies ab28289 and ab40497 were aligned with the mouse canonical sequence (http://www.uniprot.org/uniprot/P15947.fasta). The ab28289 epitope falls in a region of relatively low sequen...

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      Are these antibodies likely to cross-react with mouse proteins?

      Thank you for contacting us. I am still waiting for our source for the kallikrein antibodies ab28289 and ab40497 to send their alignments of the immunogens and mouse kallikrein but I have checked the homology of the immunogens for the other four antibo...

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