Anti-Kallikrein 1 antibody - Catalytic domain (ab40947)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab40947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 29 kDa. Dilution optimised using Chromogenic detection. A range of different sized bands can be seen for Kallikrein 1 in different tissues and in cell culture media, ranging from 16-38 kDa and likely representing different proteolytically processed forms. SDS-PAGE: Use at an assay dependent dilution. Extensive glycosylation and other posttranslational modifications give Kallikrein an apparent mass of 36-46 kDa on SDS PAGE gels. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionGlandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
    • Tissue specificityIsoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84.
    • Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
      Contains 1 peptidase S1 domain.
    • Post-translational
      modifications
      The O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located.
    • Target information above from: UniProt accession P06870 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • Glandular kallikrein 1 antibody
      • hK 1 antibody
      • hK1 antibody
      • hK1 antibody
      • Kallikrein serine protease 1 antibody
      • Kallikrein-1 antibody
      • Kidney/pancreas/salivary gland kallikrein antibody
      • Kidney/pancreas/salivary gland kallikrein antibody
      • Klk 6 antibody
      • KLK1 antibody
      • KLK1_HUMAN antibody
      • Klk6 antibody
      • KLKR antibody
      • Tissue kallikrein antibody
      see all

    Anti-Kallikrein 1 antibody - Catalytic domain images

    • All lanes : Anti-Kallikrein 1 antibody - Catalytic domain (ab40947) at 1/1000 dilution

      Lane 1 : Kallikrein 1 at 0.05 µg
      Lane 2 : Kallikrein 1 at 0.01 µg
      Lane 3 : Kallikrein 1 at 0.001 µg


      Predicted band size : 29 kDa
      Observed band size : 38,46 kDa (why is the actual band size different from the predicted?)

    References for Anti-Kallikrein 1 antibody - Catalytic domain (ab40947)

    ab40947 has not yet been referenced specifically in any publications.

    Product Wall

    Following up my previous message, immunogens for the kallikrein antibodies ab28289 and ab40497 were aligned with the mouse canonical sequence (http://www.uniprot.org/uniprot/P15947.fasta). The ab28289 epitope falls in a region of relatively low seq...

    Read More

    Thank you for contacting us. I am still waiting for our source for the kallikrein antibodies ab28289 and ab40497 to send their alignments of the immunogens and mouse kallikrein but I have checked the homology of the immunogens for the other four ant...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"