Anti-Tissue kallikrein antibody (ab169291)
Key features and details
- Mouse polyclonal to Tissue kallikrein
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Tissue kallikrein antibody
See all Tissue kallikrein primary antibodies -
Description
Mouse polyclonal to Tissue kallikrein -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Baboon, Cynomolgus monkey -
Immunogen
Recombinant full length protein within Human Tissue kallikrein aa 1-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_002248.1 -
Positive control
- IHC-P: Human salivary gland tissue. WB: Tissue kallikrein transfected 293T cell line.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.
|
|
IHC-P |
Use a concentration of 3 µg/ml.
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. |
IHC-P
Use a concentration of 3 µg/ml. |
Target
-
Function
Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. -
Tissue specificity
Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84. -
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located. - Information by UniProt
-
Database links
- Entrez Gene: 101004790 Baboon
- Entrez Gene: 102135781 Cynomolgus monkey
- Entrez Gene: 3816 Human
- Omim: 147910 Human
- SwissProt: Q07276 Cynomolgus monkey
- SwissProt: P06870 Human
- Unigene: 123107 Human
-
Alternative names
- Glandular kallikrein 1 antibody
- hK 1 antibody
- hK1 antibody
see all
Images
-
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human salivary gland tissue, labelling Tissue kallikrein with ab169291 at 3 µg/ml.
-
All lanes : Anti-Tissue kallikrein antibody (ab169291) at 1 µg/ml
Lane 1 : Tissue kallikrein transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 15 µl per lane.
Predicted band size: 29 kDa
Protocols
Datasheets and documents
-
Datasheet download
References (0)
ab169291 has not yet been referenced specifically in any publications.