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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-Kallikrein 1 antibody
See all Kallikrein 1 products (5) ...
Description
Mouse monoclonal to Kallikrein 1
Tested applications
Cross reactivity
Reacts with
Recombinant Fragment
Predicted to work with
Human
Immunogen
Recombinant fragment: FPDDLQCVDL KILPNDECKK VHVQKVTDFM LCVGHLEGGK DTCVGDSGGP LMCDGVLQGV TSWGYVPCGT PNKPSVAVRV LSYVKWIEDT IAENS, corresponding to amino acids 168-262 of Human Kallikrein 1
FPDDLQCVDL KILPNDECKK VHVQKVTDFM LCVGHLEGGK DTCVGDSGGP LMCDGVLQGV TSWGYVPCGT PNKPSVAVRV LSYVKWIEDT IAENS
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> Kallikreins
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
Cardiovascular >> Blood >> Coagulation >> Intrinsic
Cardiovascular >> Blood >> Serum Proteins
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Western blot - Kallikrein 1 antibody (ab55385)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab55385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Tissue specificity
Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84.
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.
Post-translational
modifications
The O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located.
Target information above from: UniProt accessionP06870
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- hK1 antibody
- Kidney/pancreas/salivary gland kallikrein antibody
- Glandular kallikrein 1 antibody
see all
Database links
- Entrez Gene: 3816 Human
- Omim: 147910 Human
- SwissProt: P06870 Human
- Unigene: 123107 Human
Anti-Kallikrein 1 antibody images:
Western blot - Kallikrein 1 antibody (ab55385)
Western blot against tagged recombinant protein immunogen using ab55385 Kallikrein 1 antibody at 1ug/ml. Predicted band size of immunogen is 33 kDa.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
References for Anti-Kallikrein 1 antibody (ab55385)
ab55385 has not yet been referenced specifically in any publications.
Publishing research using ab55385? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"