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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
Overview
Product name
Anti-Kallikrein 11 antibody
See all Kallikrein 11 products (4) ...
Description
Rabbit polyclonal to Kallikrein 11
Specificity
ab75225 detects endogenous levels of fragment of activated Kallikrein 11 resulting from cleavage adjacent to Ile54.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide derived from human Kallikrein 11.
Positive control
Extracts from HeLa cells treated with etoposide (25uM, 24hours).
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
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Western blot - Kallikrein 11 antibody (ab75225)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 31 kDa.
ELISA
ELISA: 1/10000ELISA: 1/10000
Target
Function
Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues.
Tissue specificity
Expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level).
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.
Post-translational
modifications
About 40% of KLK11 is inactivated by internal cleavage after Arg-188. This proteolytic inactivation may be effected by plasminogen.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ9UBX7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EC 3.4.21 antibody
- Hippostasin antibody
- hK11 antibody
see all
Database links
- Entrez Gene: 11012 Human
- Omim: 604434 Human
- SwissProt: Q9UBX7 Human
- Unigene: 57771 Human
Anti-Kallikrein 11 antibody images:
Western blot - Kallikrein 11 antibody (ab75225)
All lanes : Anti-Kallikrein 11 antibody (ab75225) at 1/500 dilution
Lane 1 : Extracts from HeLa cells, treated
with etoposide (25uM, 24hours)
Lane 2 : Extracts from HeLa cells, treated
with etoposide (25uM, 24hours) with Cl peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 31 kDa
Observed band size : 25 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Kallikrein 11 antibody (ab75225)
ab75225 has not yet been referenced specifically in any publications.
Publishing research using ab75225? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"