Overview

  • Product nameAnti-Kallikrein 11 antibodySee all Kallikrein 11 primary antibodies ...
  • Description
    Rabbit polyclonal to Kallikrein 11
  • Specificityab75225 detects endogenous levels of fragment of activated Kallikrein 11 resulting from cleavage adjacent to Ile54.
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from human Kallikrein 11.

  • Positive control
    • Extracts from HeLa cells treated with etoposide (25uM, 24hours).

Properties

Applications

Our Abpromise guarantee covers the use of ab75225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 31 kDa.
ELISA 1/10000.

Target

  • FunctionPossible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues.
  • Tissue specificityExpressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level).
  • Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    About 40% of KLK11 is inactivated by internal cleavage after Arg-188. This proteolytic inactivation may be effected by plasminogen.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 3.4.21 antibody
    • Hippostasin antibody
    • hK11 antibody
    • kallikrein 11 antibody
    • Kallikrein 11 isoform 1 preproprotein antibody
    • Kallikrein 11 isoform 2 precursor antibody
    • Kallikrein 11 precursor antibody
    • kallikrein related peptidase 11 antibody
    • Kallikrein-11 inactive chain 2 antibody
    • KLK 11 antibody
    • KLK11 antibody
    • KLK11_HUMAN antibody
    • MGC33060 antibody
    • Protease serine 20 trypsin like antibody
    • Protease serine trypsin like antibody
    • PRSS20 antibody
    • Serine protease 20 antibody
    • TLSP antibody
    • Trypsin like protease antibody
    • Trypsin-like protease antibody
    see all

Anti-Kallikrein 11 antibody images

  • All lanes : Anti-Kallikrein 11 antibody (ab75225) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells, treated with etoposide (25uM, 24hours)
    Lane 2 : Extracts from HeLa cells, treated with etoposide (25uM, 24hours) with Cl peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 31 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Kallikrein 11 antibody (ab75225)

ab75225 has not yet been referenced specifically in any publications.

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