Anti-Kallikrein 11 antibody (ab75225)

Overview

• Product nameAnti-Kallikrein 11 antibodySee all Kallikrein 11 primary antibodies ...
• Description
  Rabbit polyclonal to Kallikrein 11
• Specificityab75225 detects endogenous levels of fragment of activated Kallikrein 11 resulting from cleavage adjacent to Ile54.
• Tested applicationsWB, ELISA more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide derived from human Kallikrein 11.

• Positive controlExtracts from HeLa cells treated with etoposide (25uM, 24hours).

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Invasion/microenvironment
  • ECM
  • Extracellular matrix
  • Kallikreins

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Enzymes
  • Kallikreins

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteolytic enzymes
  • Serine protease
  • Kallikreins

Applications

Target

• FunctionPossible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues.
• Tissue specificityExpressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level).
• Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
  Contains 1 peptidase S1 domain.
• Post-translational
  modificationsAbout 40% of KLK11 is inactivated by internal cleavage after Arg-188. This proteolytic inactivation may be effected by plasminogen.
• Cellular localizationSecreted.

Target information above from: UniProt accession Q9UBX7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 11012 Human
  • Omim: 604434 Human
  • SwissProt: Q9UBX7 Human
  • Unigene: 57771 Human

• Alternative names
  EC 3.4.21 antibodyHippostasin antibodyhK11 antibody

  kallikrein 11 antibodyKallikrein 11 isoform 1 preproprotein antibodyKallikrein 11 isoform 2 precursor antibodyKallikrein 11 precursor antibodykallikrein related peptidase 11 antibodyKallikrein-11 inactive chain 2 antibodyKLK 11 antibodyKLK11 antibodyKLK11_HUMAN antibodyMGC33060 antibodyProtease serine 20 trypsin like antibodyProtease serine trypsin like antibodyPRSS20 antibodySerine protease 20 antibodyTLSP antibodyTrypsin like protease antibodyTrypsin-like protease antibody

  see all

Anti-Kallikrein 11 antibody images

• 

  Western blot - Kallikrein 11 antibody (ab75225)
  All lanes : Anti-Kallikrein 11 antibody (ab75225) at 1/500 dilution
  
  Lane 1 : Extracts from HeLa cells, treated with etoposide (25uM, 24hours)
  Lane 2 : Extracts from HeLa cells, treated with etoposide (25uM, 24hours) with Cl peptide at 5 µg
  
  Lysates/proteins at 5 µg per lane.
  
  
  Predicted band size : 31 kDa
  Observed band size : 25 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.