Anti-Kallikrein 14 antibody (ab232966)
Key features and details
- Rabbit polyclonal to Kallikrein 14
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-Kallikrein 14 antibody
See all Kallikrein 14 primary antibodies -
Description
Rabbit polyclonal to Kallikrein 14 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant fragment corresponding to Human Kallikrein 14 aa 1 to the C-terminus. Two N-terminal tags, His-tag and GST-tag. Expressed in E.coli.
Database link: Q9P0G3 -
Positive control
- IHC-P: Human breast cancer tissue. WB: Recombinant human KLK14 protein; Pig kidney and human lung tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232966 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. |
Target
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Function
Serine-type endopeptidase with a dual trypsin-like and chymotrypsin-like substrate specificity. May activate/inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11. May function in seminal clot liquefaction through direct cleavage of the semenogelin SEMG1 and SEMG2 and activation of KLK3. May function through desmoglein DSG1 cleavage in epidermal desquamation a process by which the most superficial corneocytes are shed from the skin surface. May be involved in several aspects of tumor progression including growth, invasion and angiogenesis. -
Tissue specificity
Highly expressed in CNS, bone marrow and fetal liver. Also expressed in breast, thyroid, kidney, colon, pancreas, spleen, prostate, uterus, small intestine, placenta and skeletal muscle. Among 40 tissues tested, the highest expression is detected in skin followed by breast and prostate (at protein level). Expressed in stratum corneum by sweat ducts and sweat glands and detected in sweat (at protein level). -
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsProteolytic cleavage of the activation peptide produces the active enzyme. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 43847 Human
- Omim: 606135 Human
- SwissProt: Q9P0G3 Human
- Unigene: 283925 Human
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Alternative names
- hK14 antibody
- Kallikrein 14 antibody
- Kallikrein like protein 6 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human liver tissue stained for Kallikrein 14 using ab232966 at 10 µg/mL in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Kallikrein 14 using ab232966 at 10 µg/mL in immunohistochemical analysis. DAB staining.
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Anti-Kallikrein 14 antibody (ab232966) at 1 µg/ml + Human lung tissue lysate
Secondary
HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml -
Anti-Kallikrein 14 antibody (ab232966) at 1 µg/ml + Pig kidney tissue lysate
Secondary
HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml -
Anti-Kallikrein 14 antibody (ab232966) at 2 µg/ml + Recombinant human Kallikrein 14 protein
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab232966 has not yet been referenced specifically in any publications.