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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab40961? Please let us know so that we can cite the reference in this datasheet
ab40961 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Kallikrein 15 antibody - Catalytic domain (ab40961)
All lanes : Anti-Kallikrein 15 antibody - Catalytic domain (ab40961) at 1/1000 dilution
Lane 1 : Cell line KLN 205.
SF
Lane 2 : Cell line KLN 205.
TNF-alpha
Lane 3 : Cell line KLN 205.
RA
Lysates/proteins at 0.01 µg per lane.
Predicted band size : 28 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Glycosylation and other post-translational modifications make Kallikrein-15 run at a higher molecular weight on SDS PAGE gels.
Immunocytochemistry/ Immunofluorescence-Kallikrein 15 antibody - Catalytic domain(ab40961)
ICC/IF image of ab40961 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40961, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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