Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
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ab28570 Kallikrein 15 antibody - Kallikrein loop ab40961 Kallikrein 15 antibody - Catalytic domain Can you provide the immunogenic peptide sequence or perform an alignment with the mouse protein and indicate the homology? |
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ANSWER: |
Thank you for your enquiry. The originator has informed us that the immunogenic peptide (ab28570) is ~ 80% homologous with mouse Kallikrein 15 and the immunogenic peptide (ab40961) is ~ 62% homologous with mouse Kallikrein 15. I hope this information helps. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-Kallikrein 15 antibody - Catalytic domain (ab40961) at 1/1000 dilution
Lane 1 : Cell line KLN 205.
SF
Lane 2 : Cell line KLN 205.
TNF-alpha
Lane 3 : Cell line KLN 205.
RA
Lysates/proteins at 0.01 µg per lane.
Predicted band size : 28 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Glycosylation and other post-translational modifications make Kallikrein-15 run at a higher molecular weight on SDS PAGE gels.
ICC/IF image of ab40961 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40961, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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